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Reviewed, UniProtKB/Swiss-Prot Q0T9X8 (NRFA_ECOL5)

Last modified June 16, 2009. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: ECP_4303
OrganismEscherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]
Taxonomic identifier362663 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity.

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity.

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 By similarity
Chain27 – 478452Cytochrome c-552 HAMAP MF_01182
PRO_0000268965

Sites

Metal binding941Iron (heme 3 axial ligand) By similarity
Metal binding1261Iron (heme 1 axial ligand) By similarity
Metal binding1641Iron (heme 2 axial ligand) By similarity
Metal binding2131Iron (heme 3 axial ligand) By similarity
Metal binding2151Calcium By similarity
Metal binding2161Calcium; via carbonyl oxygen By similarity
Metal binding2611Calcium; via carbonyl oxygen By similarity
Metal binding2631Calcium By similarity
Metal binding2751Iron (heme 5 axial ligand) By similarity
Metal binding2861Iron (heme 4 axial ligand) By similarity
Metal binding3011Iron (heme 2 axial ligand) By similarity
Metal binding3181Iron (heme 5 axial ligand) By similarity
Metal binding3931Iron (heme 4 axial ligand) By similarity
Binding site1221Heme 1 (covalent) By similarity
Binding site1251Heme 1 (covalent) By similarity
Binding site1601Heme 2 (covalent) By similarity
Binding site1631Heme 2 (covalent) By similarity
Binding site2091Heme 3 (covalent) By similarity
Binding site2121Heme 3 (covalent) By similarity
Binding site2161Substrate By similarity
Binding site2641Substrate By similarity
Binding site2821Heme 4 (covalent) By similarity
Binding site2851Heme 4 (covalent) By similarity
Binding site3141Heme 5 (covalent) By similarity
Binding site3171Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q0T9X8-1 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: ED06CDA0A5849707

FASTA47853,676
        10         20         30         40         50         60 
MTRIKINARR IFSLLIPFFF FTSVHAEQTA APATPVTVEA KNETFAPQHP DQYLSWKATS 

        70         80         90        100        110        120 
EQSERVDALA EDPRLVILWA GYPFSRDYNK PRGHAFAVTD VRETLRTGAP KNAEDGPLPM 

       130        140        150        160        170        180 
ACWSCKSPDV ARLIQKDGED GYFHGKWARG GPEIVNNLGC ADCHNTASPE FAKGKPELTL 

       190        200        210        220        230        240 
SRPYAARAME AIGKPFEKAG RFDQQSMVCG QCHVEYYFDG KNKAVKFPWD DGMKVENMEQ 

       250        260        270        280        290        300 
YYDKIAFSDW TNSLSKTPML KAQHPEYETW TAGIHGKNNV TCIDCHMPKV QNAEGKLYTD 

       310        320        330        340        350        360 
HKIGNPFDNF AQTCANCHTQ DKAALQKVVA ERKQSINDLK IKVEDQLVHA HFEAKAALDA 

       370        380        390        400        410        420 
GATEAEMKPI QDDIRHAQWR WDLAIASHGI HMHAPEEGLR MLGTAMDKAA DARTKLARLL 

       430        440        450        460        470 
ATKGITHEIQ IPDISTKEKA QQAIGLNMEQ IKAEKQDFIK TVIPQWEEQA RKNGLLSQ

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References

[1]"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536."
Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J.
Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000247 Genomic DNA. Translation: ABG72251.1.
RefSeqYP_672152.1.

3D structure databases

SMRQ0T9X8. Positions 38-478.
ModBaseSearch...

Genome annotation databases

GeneID4187306.
GenomeReviewsGene locus ECP_4303 in contig CP000247_GR.
KEGGecp:ECP_4303.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ0T9X8.
OMAQ0T9X8. CHDQSKE.

Enzyme and pathway databases

BioCycECOL362663:ECP_4303-MON.

Family and domain databases

HAMAPMF_01182.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_ECOL5
AccessionPrimary (citable) accession number: Q0T9X8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: September 5, 2006
Last modified: June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents