Reviewed, UniProtKB/Swiss-Prot Q0T9J7 (ULAD_ECOL5)

Names and origin

Protein names

Recommended name:
    3-keto-L-gulonate-6-phosphate decarboxylase ulaD
    EC=4.1.1.85
Alternative name(s):
    3-dehydro-L-gulonate-6-phosphate decarboxylase
    KGPDC
    L-ascorbate utilization protein D

Gene names

Name:	ulaD
Ordered Locus Names:	ECP_4441

Organism

Escherichia coli O6:K15:H31 (strain 536 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

362663 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the decarboxylation of 3-keto-L-gulonate-6-P into L-xylulose-5-P. Is involved in the anaerobic L-ascorbate utilization By similarity.
Catalytic activity	3-dehydro-L-gulonate 6-phosphate = L-xylulose 5-phosphate + CO2. HAMAP MF_01267
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor degradation; L-ascorbic acid degradation; D-xylulose 5-phosphate from L-ascorbic acid: step 2/4. HAMAP MF_01267
Subunit structure	Homodimer By similarity.
Induction	Induced by L-ascorbate. Repressed by ulaR By similarity.
Sequence similarities	Belongs to the HPS/KGPDC family. KGPDC subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Magnesium Metal-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro L-ascorbic acid catabolic process Inferred from electronic annotation. Source: HAMAP carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydro-L-gulonate-6-phosphate decarboxylase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	3-keto-L-gulonate-6-phosphate decarboxylase ulaD HAMAP MF_01267		PRO_1000067320
Sites
	Metal binding	33	1	Magnesium By similarity
	Metal binding	62	1	Magnesium By similarity
	Binding site	11	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Site	64	1	Transition state stabilizer By similarity
	Site	67	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0T9J7-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: EC8490DA1D02D824	FASTA	216	23,578
	10 20 30 40 50 60 MSLPMLQVAL DNQTMDSAYE TTRLIAEEVD IIEVGTILCV GEGVRAVRDL KALYPHKIVL 70 80 90 100 110 120 ADAKIADAGK ILSRMCFEAN ADWVTVICCA DINTAKGALD VAKEFNGDVQ IELTGYWTWE 130 140 150 160 170 180 QAQQWRDAGI GQVVYHRSRD AQAAGVAWGE ADITAIKRLS DMGFKVTVTG GLALEDLPLF 190 200 210 KGIPIHVFIA GRSIRDAASP VEAARQFKRS IAELWG

References

[1]

"Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536." Hochhut B., Wilde C., Balling G., Middendorf B., Dobrindt U., Brzuszkiewicz E., Gottschalk G., Carniel E., Hacker J. Mol. Microbiol. 61:584-595(2006) [PubMed: 16879640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000247 Genomic DNA. Translation: ABG72382.1.
RefSeq	YP_672283.1.
3D structure databases
SMR	Q0T9J7. Positions 2-216.
ModBase	Search...
Genome annotation databases
GeneID	4190033.
GenomeReviews	Gene locus ECP_4441 in contig CP000247_GR.
KEGG	ecp:ECP_4441.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0T9J7.
OMA	Q0T9J7. PIYIFIA.
Enzyme and pathway databases
BioCyc	ECOL362663:ECP_4441-MON.
Family and domain databases
HAMAP	MF_01267. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001754. OMPdecase_core. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00215. OMPdecase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ULAD_ECOL5

Accession

Primary (citable) accession number: Q0T9J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)