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Q0T7K9 (Q0T7K9_SHIF8) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL ABF02617.1
Synonyms:ddl HAMAP-Rule MF_00047
Ordered Locus Names:SFV_0344 EMBL ABF02617.1
OrganismShigella flexneri serotype 5b (strain 8401) [Complete proteome] [HAMAP] EMBL ABF02617.1
Taxonomic identifier373384 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain127 – 330204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding157 – 21256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2841Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2971Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2971Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2991Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
Q0T7K9 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: C1646DC14682CD96

FASTA34637,379
        10         20         30         40         50         60 
MSLQSAKNIV DAIDKSRFDV VLLGIDKQGQ WHVSDASNYL LNADDPAHIA LRPSATSLAQ 

        70         80         90        100        110        120 
VPGKHEHQLI DAQNGQPLPT VDVIFPIVHG TLGEDGSLQG MLRVANLPFV GSDVLASAAC 

       130        140        150        160        170        180 
MDKDVTKRLL RDAGLNIAPF ITLTRANRHN ISFAEVESKL GLPLFVKPAN QGSSVGVSKV 

       190        200        210        220        230        240 
TSEEQYAIAV DLAFEFDHKV IVEQGIKGRE IECAVLGNDN PQASTCGEIV LTSDFYAYDT 

       250        260        270        280        290        300 
KYIDEDGAKV VVPAAIAPEI NDKIRAIAVQ AYQTLGCAGM ARVDVFLTPE NEVVINEINT 

       310        320        330        340 
LPGFTNISMY PKLWQASGLG YTDLITRLIE LALERHAADN ALKTTM 

« Hide

References

[1]"Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a."
Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.
BMC Genomics 7:173-173(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 8401 EMBL ABF02617.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000266 Genomic DNA. Translation: ABF02617.1.
RefSeqYP_687922.1. NC_008258.1.

3D structure databases

ProteinModelPortalQ0T7K9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING373384.SFV_0344.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABF02617; ABF02617; SFV_0344.
GeneID4209179.
KEGGsfv:SFV_0344.
PATRIC18723677. VBIShiFle33408_0387.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycSFLE373384:GHZM-343-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ0T7K9_SHIF8
AccessionPrimary (citable) accession number: Q0T7K9
Entry history
Integrated into UniProtKB/TrEMBL: September 5, 2006
Last sequence update: September 5, 2006
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)