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Q0T6Q2

- SYL_SHIF8

UniProt

Q0T6Q2 - SYL_SHIF8

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Protein
Leucine--tRNA ligase
Gene
leuS, SFV_0684
Organism
Shigella flexneri serotype 5b (strain 8401)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSFLE373384:GHZM-683-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:SFV_0684
OrganismiShigella flexneri serotype 5b (strain 8401)
Taxonomic identifieri373384 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000000659: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 860860Leucine--tRNA ligaseUniRule annotation
PRO_0000334820Add
BLAST

Proteomic databases

PRIDEiQ0T6Q2.

Interactioni

Protein-protein interaction databases

STRINGi373384.SFV_0684.

Structurei

3D structure databases

ProteinModelPortaliQ0T6Q2.
SMRiQ0T6Q2. Positions 228-413.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionUniRule annotation
Add
BLAST
Motifi619 – 6235"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0T6Q2-1 [UniParc]FASTAAdd to Basket

« Hide

MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM    50
GHVRNYTIGD VIARYQHMLG KNVLQPIGWD AFGLPAEGAA VKHHTAPAPW 100
TYDNIAYMKN QLKMLGFGYD WSRELATCTP EYYRWEQKFF TELYKKGLVY 150
KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD TKVERKEIPQ WFIKITAYAD 200
ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD NTLTVYTTRP 250
DTFMGCTYLA VAAGHPLAQK AAENNPELAA FIDECRNTKV AEAEMATMEK 300
KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS 350
KYGLNIKPVI LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA 400
DKLTAMGVGE RKVNYRLRDW GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL 450
PVILPEDVVM DGITSPIKAD PEWAKTTVNG MPALRETDTF DTFMESSWYY 500
ARYTCPEYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY FRFFHKLMRD 550
AGMVNSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR 600
IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS 650
PADMTLEWQE SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTEDQKA 700
LRRDVHKTIA KVTDDIGRRQ TFNTAIAAIM ELMNKLAKAP TDGEQDRALM 750
QEALQAVVRM LNPFTPHICF TLWQELKGEG DIDNAPWPVA DEKAMVEDST 800
LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD GVTVRKVIYV 850
PGKLLNLVVG 860
Length:860
Mass (Da):97,278
Last modified:May 20, 2008 - v2
Checksum:iA3DD5489FE142518
GO

Sequence cautioni

The sequence ABF02924.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000266 Genomic DNA. Translation: ABF02924.1. Different initiation.
RefSeqiYP_688229.1. NC_008258.1.

Genome annotation databases

EnsemblBacteriaiABF02924; ABF02924; SFV_0684.
GeneIDi4209927.
KEGGisfv:SFV_0684.
PATRICi18724479. VBIShiFle33408_0773.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000266 Genomic DNA. Translation: ABF02924.1 . Different initiation.
RefSeqi YP_688229.1. NC_008258.1.

3D structure databases

ProteinModelPortali Q0T6Q2.
SMRi Q0T6Q2. Positions 228-413.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 373384.SFV_0684.

Proteomic databases

PRIDEi Q0T6Q2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABF02924 ; ABF02924 ; SFV_0684 .
GeneIDi 4209927.
KEGGi sfv:SFV_0684.
PATRICi 18724479. VBIShiFle33408_0773.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200747.
KOi K01869.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci SFLE373384:GHZM-683-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a."
    Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.
    BMC Genomics 7:173-173(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 8401.

Entry informationi

Entry nameiSYL_SHIF8
AccessioniPrimary (citable) accession number: Q0T6Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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