Reviewed, UniProtKB/Swiss-Prot Q0T5R7 (NAGK_SHIF8)

Names and origin

Protein names

Recommended name:
    N-acetyl-D-glucosamine kinase
    EC=2.7.1.59
Alternative name(s):
    GlcNAc kinase

Gene names

Name:	nagK
Ordered Locus Names:	SFV_1139

Organism

Shigella flexneri serotype 5b (strain 8401) [Complete proteome] [HAMAP]

Taxonomic identifier

373384 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P By similarity.
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate. HAMAP MF_01271
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_01271
Sequence similarities	Belongs to the ROK (nagC/xylR) family. NagK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: HAMAP peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	N-acetyl-D-glucosamine kinase HAMAP MF_01271		PRO_0000301942
Regions
	Nucleotide binding	4 – 11	8	ATP Potential
	Nucleotide binding	133 – 140	8	ATP Potential
Sites
	Metal binding	157	1	Zinc By similarity
	Metal binding	177	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	184	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0T5R7-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 65572B2CCFEBFBA5	FASTA	303	32,987
	10 20 30 40 50 60 MYYGFDIGGT KIALGVFDSG RQLQWEKRVP TPRDSYDAFL DAVCELVAEA DQRFGCKGSV 70 80 90 100 110 120 GIGIPGMPET EDGKLYAANV PAASGKPLRA DLSARLDRDV RLDNDANCFA LSEAWDDEFT 130 140 150 160 170 180 QYPLVMGLIL GTGVGGGLVF NGKPITGKSY ITGEFGHMRL PVDALTMMGL DFPLRRCGCG 190 200 210 220 230 240 QHGCIENYLS GRGFAWLYQH YYHQPLQAPE IIALYDQGDE QARAHVERYL DLLAVSLGNI 250 260 270 280 290 300 LTIVDPDLVV IGGGLSNFPA ITTQLADRLP CHLLPVARVP RIERARHGDA GGMRGAAFLH LTD

References

[1]

"Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a." Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q. BMC Genomics 7:173-173(2006) [PubMed: 16822325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000266 Genomic DNA. Translation: ABF03348.1.
RefSeq	YP_688653.1.
3D structure databases
SMR	Q0T5R7. Positions 1-303.
ModBase	Search...
Genome annotation databases
GeneID	4208953.
GenomeReviews	Gene locus SFV_1139 in contig CP000266_GR.
KEGG	sfv:SFV_1139.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0T5R7.
OMA	Q0T5R7. HVERFME.
Enzyme and pathway databases
BioCyc	SFLE373384:SFV_1139-MON.
Family and domain databases
HAMAP	MF_01271. [Tree]
InterPro	IPR000600. ROK. [Graphical view]
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGK_SHIF8

Accession

Primary (citable) accession number: Q0T5R7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)