ID   GATY_SHIF8              Reviewed;         284 AA.
AC   Q0T342;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 2.
DT   16-JUN-2009, entry version 19.
DE   RecName: Full=D-tagatose-1,6-bisphosphate aldolase subunit gatY;
DE            Short=TagBP aldolase;
DE            Short=TBPA;
DE            EC=4.1.2.40;
DE   AltName: Full=Tagatose-bisphosphate aldolase;
DE   AltName: Full=D-tagatose-bisphosphate aldolase class II;
GN   Name=gatY; OrderedLocusNames=SFV_2151;
OS   Shigella flexneri serotype 5b (strain 8401).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=373384;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16822325; DOI=10.1186/1471-2164-7-173;
RA   Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z.,
RA   Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y.,
RA   Jin Q.;
RT   "Complete genome sequence of Shigella flexneri 5b and comparison with
RT   Shigella flexneri 2a.";
RL   BMC Genomics 7:173-173(2006).
CC   -!- FUNCTION: Catalytic subunit of the tagatose-1,6-bisphosphate
CC       aldolase gatYZ, which catalyzes the reversible aldol condensation
CC       of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with
CC       glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6-
CC       bisphosphate (TBP). Requires gatZ subunit for full activity and
CC       stability. Is involved in the catabolism of galactitol (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: D-tagatose 1,6-bisphosphate = glycerone
CC       phosphate + D-glyceraldehyde 3-phosphate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate metabolism; D-tagatose 6-phosphate
CC       degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate
CC       from D-tagatose 6-phosphate: step 2/2.
CC   -!- SUBUNIT: Forms a complex with gatZ (By similarity).
CC   -!- SIMILARITY: Belongs to the class II fructose-bisphosphate aldolase
CC       family. TagBP aldolase gatY subfamily.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CP000266; ABF04273.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_689578.2; -.
DR   GeneID; 4207161; -.
DR   GenomeReviews; CP000266_GR; SFV_2151.
DR   KEGG; sfv:SFV_2151; -.
DR   HOGENOM; Q0T342; -.
DR   BioCyc; SFLE373384:SFV_2151-MON; -.
DR   GO; GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:InterPro.
DR   GO; GO:0009025; F:tagatose-bisphosphate aldolase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019404; P:galactitol catabolic process; IEA:HAMAP.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0019512; P:lactose catabolic process via tagatose-6-ph...; IEA:InterPro.
DR   HAMAP; MF_01294; -; 1.
DR   InterPro; IPR000771; Ketose_bisP_aldolase_II.
DR   InterPro; IPR011288; Tag_bisphos_ald.
DR   Pfam; PF01116; F_bP_aldolase; 1.
DR   ProDom; PD002376; K_bP_aldolase; 1.
DR   TIGRFAMs; TIGR00167; cbbA; 1.
DR   TIGRFAMs; TIGR01858; tag_bisphos_ald; 1.
DR   PROSITE; PS00602; ALDOLASE_CLASS_II_1; 1.
DR   PROSITE; PS00806; ALDOLASE_CLASS_II_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Galactitol metabolism; Lyase; Metal-binding; Zinc.
FT   CHAIN         1    284       D-tagatose-1,6-bisphosphate aldolase
FT                                subunit gatY.
FT                                /FTId=PRO_0000355355.
FT   REGION      209    211       Dihydroxyacetone phosphate binding (By
FT                                similarity).
FT   REGION      230    233       Dihydroxyacetone phosphate binding (By
FT                                similarity).
FT   ACT_SITE     82     82       Proton donor (By similarity).
FT   METAL        83     83       Zinc; catalytic (By similarity).
FT   METAL       180    180       Zinc; catalytic (By similarity).
FT   METAL       208    208       Zinc; catalytic (By similarity).
FT   BINDING     181    181       Dihydroxyacetone phosphate; via amide
FT                                nitrogen (By similarity).
SQ   SEQUENCE   284 AA;  30914 MW;  D861B375ACE51CA0 CRC64;
     MYVVSTKQML NNAQRGGYAV PAFNIHNLET MQVVVETAAN LHAPVIIAGT PGTFTHAGTE
     NLLALVSAMA KQYHHPLAIH LDHHTKFDDI AQKVRSGVRS VMIDASHLPF AQNISRVKEV
     VDFCHRFDVS VEAELGQLGG QEDDVQVNEV DALYTNPAQA REFAEATGID SLAVTIGTAH
     GMYASAPVLD FSRLENIRQW VNLPLVLHGA SGLSTKDIQQ TIKLGICKIN VATELKNAFS
     QSLKNYLTEH PEATDPRDYL QSAKSAMRDV VSKVIADCGC EGRA
//