Q0T1Y0 (HCAF_SHIF8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phenylpropionate/cinnamic acid dioxygenase subunit beta
EC=1.14.12.19

Alternative name(s):
Digoxigenin subunit beta

Gene names

Name:	hcaF
Ordered Locus Names:	SFV_2587

Organism

Shigella flexneri serotype 5b (strain 8401) [Complete proteome] [HAMAP]

Taxonomic identifier

373384 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively By similarity. HAMAP MF_01649
Catalytic activity	3-phenylpropanoate + NADH + O2 = 3-(cis-5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+. (2E)-3-phenylprop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + NAD+.
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01649
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (hcaE and hcaF), a ferredoxin (hcaC) and a ferredoxin reductase (hcaD) By similarity.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-phenylpropionate dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 172	172	3-phenylpropionate/cinnamic acid dioxygenase subunit beta HAMAP MF_01649		PRO_0000333716

Sequences

	Sequence		Length	Mass (Da)
	Q0T1Y0 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 582027F378D97554	FASTA	172	20,613
	10 20 30 40 50 60 MSAQVSLELH HRISQFLFHE ASLLDDWKFR DWLAQLDEEI RYTMRTTVNA QTRDRRKGVQ 70 80 90 100 110 120 PPTTWIFNDT KDQLERRIAR LETGMAWAEE PPSRTRHLIS NCQVSETDIP NVFAVRVNYL 130 140 150 160 170 LYRAQKERDE TFYVGTRFDK VRRLEYDNWR LLERDIVLDQ AVITSHNLSV LF

References

[1]

"Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a." Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q. BMC Genomics 7:173-173(2006) [PubMed: 16822325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 8401.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000266 Genomic DNA. Translation: ABF04685.1.
RefSeq	YP_689990.1. NC_008258.1.
3D structure databases
ProteinModelPortal	Q0T1Y0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0T1Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000097500; EBESCP00000093696; EBESCG00000096544.
GeneID	4207322.
GenomeReviews	Gene locus SFV_2587 in contig CP000266_GR.
KEGG	sfv:SFV_2587.
PATRIC	18728982. VBIShiFle33408_2976.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG5517.
GeneTree	EBGT00050000011518.
HOGENOM	HBG582498.
OMA	DIFAGER.
ProtClustDB	PRK10069.
Enzyme and pathway databases
BioCyc	SFLE373384:SFV_2587-MONOMER.
Family and domain databases
HAMAP	MF_01649. HcaF. [Tree]
InterPro	IPR023712. HcaF. IPR000391. Rng_hydr_dOase-bsu. [Graphical view]
KO	K05709.
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCAF_SHIF8

Accession

Primary (citable) accession number: Q0T1Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program