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Protein

Adenylyl-sulfate kinase

Gene

cysC

Organism
Shigella flexneri serotype 5b (strain 8401)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of activated sulfate.UniRule annotation

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 2 (cysD), Sulfate adenylyltransferase subunit 1 (cysN)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Phosphoserine intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 428ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSFLE373384:GHZM-2744-MONOMER.
UniPathwayiUPA00140; UER00205.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl-sulfate kinaseUniRule annotation (EC:2.7.1.25UniRule annotation)
Alternative name(s):
APS kinaseUniRule annotation
ATP adenosine-5'-phosphosulfate 3'-phosphotransferaseUniRule annotation
Adenosine-5'-phosphosulfate kinaseUniRule annotation
Gene namesi
Name:cysCUniRule annotation
Ordered Locus Names:SFV_2748
OrganismiShigella flexneri serotype 5b (strain 8401)
Taxonomic identifieri373384 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000000659 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201Adenylyl-sulfate kinasePRO_1000009033Add
BLAST

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ0T1I1.
SMRiQ0T1I1. Positions 26-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the APS kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228204.
KOiK00860.
OMAiNIKWHNL.
OrthoDBiEOG6GR38Q.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0T1I1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALHDENVVW HSHPVTVQQR ELHHGHRGVV LWFTGLSGSG KSTVAGALEE
60 70 80 90 100
ALHKLGVSTY LLDGDNVRHG LCSDLGFSDA DRKENIRRVG EVANLMVEAG
110 120 130 140 150
LVVLTAFISP HRAERQMVRE RVGEGRFIEV FVDTPLAICE ARDPKGLYKK
160 170 180 190 200
ARAGELRNFT GIDSVYEAPE SAEIHLNGEQ LVTNLVQQLL DLLRQNDIIR

S
Length:201
Mass (Da):22,321
Last modified:September 5, 2006 - v1
Checksum:i11E15BB8F9D2FD4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000266 Genomic DNA. Translation: ABF04834.1.
RefSeqiWP_001173673.1. NC_008258.1.

Genome annotation databases

EnsemblBacteriaiABF04834; ABF04834; SFV_2748.
KEGGisfv:SFV_2748.
PATRICi18729353. VBIShiFle33408_3153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000266 Genomic DNA. Translation: ABF04834.1.
RefSeqiWP_001173673.1. NC_008258.1.

3D structure databases

ProteinModelPortaliQ0T1I1.
SMRiQ0T1I1. Positions 26-179.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF04834; ABF04834; SFV_2748.
KEGGisfv:SFV_2748.
PATRICi18729353. VBIShiFle33408_3153.

Phylogenomic databases

HOGENOMiHOG000228204.
KOiK00860.
OMAiNIKWHNL.
OrthoDBiEOG6GR38Q.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00205.
BioCyciSFLE373384:GHZM-2744-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a."
    Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.
    BMC Genomics 7:173-173(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 8401.

Entry informationi

Entry nameiCYSC_SHIF8
AccessioniPrimary (citable) accession number: Q0T1I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: May 11, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.