Q0T157 (FUCI_SHIF8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-fucose isomerase
EC=5.3.1.25

Alternative name(s):
6-deoxy-L-galactose isomerase
FucIase

Gene names

Name:	fucI
Ordered Locus Names:	SFV_2881

Organism

Shigella flexneri serotype 5b (strain 8401) [Complete proteome] [HAMAP]

Taxonomic identifier

373384 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella › Shigella flexneri › Shigella flexneri 5

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts the aldose L-fucose into the corresponding ketose L-fuculose By similarity. HAMAP-Rule MF_01254
Catalytic activity	L-fucose = L-fuculose. HAMAP-Rule MF_01254
Cofactor	Manganese By similarity.
Pathway	Carbohydrate degradation; L-fucose degradation; L-lactaldehyde and glycerone phosphate from L-fucose: step 1/3. HAMAP-Rule MF_01254
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-fucose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Fucose metabolism
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fucose catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-fucose isomerase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	L-fucose isomerase HAMAP-Rule MF_01254		PRO_1000067226
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	361	1	Proton acceptor By similarity
	Metal binding	337	1	Manganese By similarity
	Metal binding	361	1	Manganese By similarity
	Metal binding	528	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0T157 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: E6245DEEDF34EF9B	FASTA	591	64,977
	10 20 30 40 50 60 MKKISLPKIG IRPVIDGRRM GVRESLEEQT MNMAKATAAL LTEKLRHACG AAVECVISDT 70 80 90 100 110 120 CIAGMAEAAA CEEKFSSQNV GLTITVTPCW CYGSETIDMD PTRPKAIWGF NGTERPGAVY 130 140 150 160 170 180 LAAALAAHSQ KGIPAFSIYG HDVQDADDTS IPADVEEKLL RFARAGLAVA SMKGKSYLSL 190 200 210 220 230 240 GGVSMGIAGS IVDHNFFESW LGMKVQAVDM TELRRRIDQK IYDEAELEMA LAWADKNFRY 250 260 270 280 290 300 GEDENNKQYQ RNAEQSRAVL RESLLMAMCI RDMMQGNSKL ADIGRVEESL GYNAIAAGFQ 310 320 330 340 350 360 GQRHWTDQYP NGDTAEAILN SSFDWNGVRE PFVVATENDS LNGVAMLMGH QLTGTAQVFA 370 380 390 400 410 420 DVRTYWSPEA IERVTGHKLD GLAEHGIIHL INSGSAALDG SCKQRDSEGN PTMKPHWEIS 430 440 450 460 470 480 QQEADACLAA TEWCPAIHEY FRGGGYSSRF LTEGGVPFTM TRVNIIKGLG PVLQIAEGWS 490 500 510 520 530 540 VELPKDVHDI LNKRTNSTWP TTWFAPRLTG KGPFTDVYSV MANWGANHGV LTIGHVGADF 550 560 570 580 590 ITLASMLRIP VCMHNVEETK VYRPSAWAAH GMDIEGQDYR ACQNYGPLYK R

References

[1]

"Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a." Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q. BMC Genomics 7:173-173(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 8401.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000266 Genomic DNA. Translation: ABF04958.1.
RefSeq	YP_690263.1. NC_008258.1.
3D structure databases
ProteinModelPortal	Q0T157.
SMR	Q0T157. Positions 1-591.
ModBase	Search...
Protein-protein interaction databases
STRING	373384.SFV_2881.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABF04958; ABF04958; SFV_2881.
GeneID	4210301.
KEGG	sfv:SFV_2881.
PATRIC	18729653. VBIShiFle33408_3298.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2407.
HOGENOM	HOG000249674.
KO	K01818.
OMA	KIGIRPT.
ProtClustDB	PRK10991.
Enzyme and pathway databases
BioCyc	SFLE373384:GHZM-2927-MONOMER.
UniPathway	UPA00563; UER00624.
Family and domain databases
Gene3D	3.20.14.10. 1 hit. 3.40.275.10. 1 hit. 3.40.50.1070. 1 hit.
HAMAP	MF_01254. Fucose_iso.
InterPro	IPR004216. Fuc/Ara_isomerase_C. IPR015888. Fuc_isomerase_C. IPR012888. Fucose_iso_N1. IPR005763. Fucose_isomerase. IPR009015. Fucose_isomerase_N/cen. IPR012889. Fucose_isomerase_N2. [Graphical view]
Pfam	PF02952. Fucose_iso_C. 1 hit. PF07881. Fucose_iso_N1. 1 hit. PF07882. Fucose_iso_N2. 1 hit. [Graphical view]
SUPFAM	SSF50443. Fuc_isomerase_C. 1 hit. SSF53743. Fuc_isomerase_N. 1 hit.
TIGRFAMs	TIGR01089. fucI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FUCI_SHIF8

Accession

Primary (citable) accession number: Q0T157

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program