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Q0T0S8

- TRMB_SHIF8

UniProt

Q0T0S8 - TRMB_SHIF8

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
trmB, SFV_3018
Organism
Shigella flexneri serotype 5b (strain 8401)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691S-adenosyl-L-methionine By similarity
Binding sitei94 – 941S-adenosyl-L-methionine By similarity
Binding sitei121 – 1211S-adenosyl-L-methionine By similarity
Active sitei144 – 1441 By similarity
Binding sitei144 – 1441S-adenosyl-L-methionine By similarity
Binding sitei148 – 1481Substrate By similarity
Binding sitei180 – 1801Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSFLE373384:GHZM-3014-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:SFV_3018
OrganismiShigella flexneri serotype 5b (strain 8401)
Taxonomic identifieri373384 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000000659: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_0000288228Add
BLAST

Proteomic databases

PRIDEiQ0T0S8.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi373384.SFV_3018.

Structurei

3D structure databases

ProteinModelPortaliQ0T0S8.
SMRiQ0T0S8. Positions 36-239.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1556Interaction with RNA Reviewed prediction
Regioni217 – 2204Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0T0S8-1 [UniParc]FASTAAdd to Basket

« Hide

MKNDVISPEF DENGRPLRRI RSFVRRQGRL TKGQEHALEN YWPVMGVEFS    50
EDMLDFPALF GREAPVTLEI GFGMGASLVA MAKDRPEQDF LGIEVHSPGV 100
GACLASAHEE GLSNLRVMCH DAVEVLHKMI PDNSLRMVQL FFPDPWHKAR 150
HNKRRIVQVP FAELVKSKLQ LGGVFHMATD WEPYAEHMLE VMSSIDGYKN 200
LSESNDYVPR PASRPVTKFE QRGHRLGHGV WDLMFERVK 239
Length:239
Mass (Da):27,307
Last modified:September 5, 2006 - v1
Checksum:iB4C8AF9A1EF85FEB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000266 Genomic DNA. Translation: ABF05087.1.
RefSeqiYP_690392.1. NC_008258.1.

Genome annotation databases

EnsemblBacteriaiABF05087; ABF05087; SFV_3018.
GeneIDi4210765.
KEGGisfv:SFV_3018.
PATRICi18729973. VBIShiFle33408_3454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000266 Genomic DNA. Translation: ABF05087.1 .
RefSeqi YP_690392.1. NC_008258.1.

3D structure databases

ProteinModelPortali Q0T0S8.
SMRi Q0T0S8. Positions 36-239.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 373384.SFV_3018.

Proteomic databases

PRIDEi Q0T0S8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABF05087 ; ABF05087 ; SFV_3018 .
GeneIDi 4210765.
KEGGi sfv:SFV_3018.
PATRICi 18729973. VBIShiFle33408_3454.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci SFLE373384:GHZM-3014-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a."
    Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.
    BMC Genomics 7:173-173(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 8401.

Entry informationi

Entry nameiTRMB_SHIF8
AccessioniPrimary (citable) accession number: Q0T0S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: September 5, 2006
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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