Q0T068 (NANK_SHIF8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmannosamine kinase
EC=2.7.1.60

Alternative name(s):
ManNAc kinase
N-acetyl-D-mannosamine kinase

Gene names

Name:	nanK
Ordered Locus Names:	SFV_3247

Organism

Shigella flexneri serotype 5b (strain 8401) [Complete proteome] [HAMAP]

Taxonomic identifier

373384 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P By similarity. HAMAP MF_01234
Catalytic activity	ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate. HAMAP MF_01234
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 2/5. HAMAP MF_01234
Subunit structure	Homodimer By similarity. HAMAP MF_01234
Sequence similarities	Belongs to the ROK (nagC/xylR) family. NanK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylneuraminate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acylmannosamine kinase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	N-acetylmannosamine kinase HAMAP MF_01234		PRO_0000301461
Regions
	Nucleotide binding	5 – 12	8	ATP Potential
	Nucleotide binding	132 – 139	8	ATP Potential
Sites
	Metal binding	156	1	Zinc By similarity
	Metal binding	166	1	Zinc By similarity
	Metal binding	168	1	Zinc By similarity
	Metal binding	173	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0T068 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 1F180C9C589FC1E7	FASTA	291	29,698
	10 20 30 40 50 60 MTILAIDIGG TKLAAALIGA DGQIRDRREL PTPASQTPEA LRDALSALVS PLQAHAQRVA 70 80 90 100 110 120 IASTGIIRDG SLLALNPHNL GGLLHFPLVK TLEQLTNLPT IAINDAQAAA WAEYQALEGD 130 140 150 160 170 180 ITDMVFITVS TGVGGGVVSG GKLLTGPGGL AGHIGHTLAD PHGPVCGCGR TGCVEAIASG 190 200 210 220 230 240 RGIAAAAQGE LAGADARTIF TRAGQGDEQA QQLIHRSART LARLIADIKA TTDCQCVVVG 250 260 270 280 290 GSVGLAEGYL ALVEMYLAQE PAAFHVDLLA AHYRHDAGLL GAALLAQGEK L

References

[1]

"Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a." Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q. BMC Genomics 7:173-173(2006) [PubMed: 16822325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 8401.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000266 Genomic DNA. Translation: ABF05297.1.
RefSeq	YP_690602.1. NC_008258.1.
3D structure databases
ProteinModelPortal	Q0T068.
SMR	Q0T068. Positions 1-289.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0T068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000097420; EBESCP00000093616; EBESCG00000096464.
GeneID	4209285.
GenomeReviews	Gene locus SFV_3247 in contig CP000266_GR.
KEGG	sfv:SFV_3247.
PATRIC	18730501. VBIShiFle33408_3714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1940.
GeneTree	EBGT00050000009665.
HOGENOM	HBG730260.
OMA	LRISQCR.
ProtClustDB	PRK05082.
Enzyme and pathway databases
BioCyc	SFLE373384:SFV_3247-MONOMER.
Family and domain databases
HAMAP	MF_01234. ManNAc_kinase. [Tree]
InterPro	IPR023945. ManNAc_kinase_bac. IPR000600. ROK. [Graphical view]
KO	K00885.
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NANK_SHIF8

Accession

Primary (citable) accession number: Q0T068

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program