Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q0SZE5 (GHRB_SHIF8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glyoxylate/hydroxypyruvate reductase B

EC=1.1.1.79
EC=1.1.1.81
Gene names
Name:ghrB
Ordered Locus Names:SFV_3534
OrganismShigella flexneri serotype 5b (strain 8401) [Complete proteome] [HAMAP]
Taxonomic identifier373384 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01667

Catalytic activity

Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667

D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667

Subunit structure

Homodimer By similarity. HAMAP MF_01667

Subcellular location

Cytoplasm By similarity HAMAP MF_01667.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD binding

Inferred from electronic annotation. Source: InterPro

glyoxylate reductase (NADP) activity

Inferred from electronic annotation. Source: EC

hydroxypyruvate reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667
PRO_0000348403

Sites

Active site2371 By similarity
Active site2661 By similarity
Active site2851Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0SZE5 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 7A19271D6AFAC79E

FASTA32435,337
        10         20         30         40         50         60 
MKPSVILYKA LPDDLLQRLQ AHFTVHQVAN LSPQTVVQNA AIFAEAEGLL GSNENVDAAL 

        70         80         90        100        110        120 
LEKMPKLRAT STISVGYDNF DVDALTARKI LLMHTPTVLT ETVADTLMAL VLSTARRVVE 

       130        140        150        160        170        180 
VAERVKAGEW TASIGPDWYG TDVHHKTLGI VGMGRIGMAL AQRAHFGFNM PILYNARRHH 

       190        200        210        220        230        240 
KEAEERFNAR YCDLDTLLQE SDFVCLILPL TDETHHLFGA EQFAKMKSSA IFINAGRGPV 

       250        260        270        280        290        300 
VDENALIAAL QKGEIHVAGL DVFEQEPLSV DSPLLSMANV VAVPHIGSAT HETRYGMAAC 

       310        320 
AVDNLIDALQ GKVEKNCVNP HVAD 

« Hide

References

[1]"Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a."
Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q.
BMC Genomics 7:173-173(2006) [PubMed: 16822325] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 8401.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000266 Genomic DNA. Translation: ABF05570.1.
RefSeqYP_690875.1. NC_008258.1.

3D structure databases

ProteinModelPortalQ0SZE5.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0SZE5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000098122; EBESCP00000094318; EBESCG00000097166.
GeneID4208319.
GenomeReviewsGene locus SFV_3534 in contig CP000266_GR.
KEGGsfv:SFV_3534.
PATRIC18731136. VBIShiFle33408_4025.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1052.
GeneTreeEBGT00050000009079.
HOGENOMHBG731446.
OMAHALTEKK.
ProtClustDBPRK15409.

Enzyme and pathway databases

BioCycSFLE373384:SFV_3534-MONOMER.

Family and domain databases

HAMAPMF_01667. 2-Hacid_dh_C_GhrB.
[Tree]
InterProIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR023756. Glyo/OHPyrv_Rdtase_B.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KOK00090.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEPS00065. D_2_HYDROXYACID_DH_1. False negative.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGHRB_SHIF8
AccessionPrimary (citable) accession number: Q0SZE5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 5, 2006
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families