Q0SYW9 (GPPA_SHIF8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase
EC=3.6.1.40

Alternative name(s):
Guanosine pentaphosphate phosphohydrolase
pppGpp-5'-phosphohydrolase

Gene names

Name:	gppA
Ordered Locus Names:	SFV_3725

Organism

Shigella flexneri serotype 5b (strain 8401) [Complete proteome] [HAMAP]

Taxonomic identifier

373384 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in the coordinated regulation of numerous cellular activities By similarity. HAMAP MF_01550
Catalytic activity	Guanosine 5'-triphosphate,3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate. HAMAP MF_01550
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2. HAMAP MF_01550
Sequence similarities	Belongs to the gppA/ppx family. GppA subfamily.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	guanosine pentaphosphate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	guanosine-5'-triphosphate,3'-diphosphate diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase HAMAP MF_01550		PRO_0000314499

Sequences

	Sequence		Length	Mass (Da)
	Q0SYW9 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: DE8D75405A41CF5E	FASTA	494	54,972
	10 20 30 40 50 60 MGSTSSLYAA IDLGSNSFHM LVVREVAGSI QTLTRIKRKV RLAAGLNSEN DLSNEAMERG 70 80 90 100 110 120 WQCLRLFAER LQDIPPSQIR VVATATLRLA VNAGDFIAKA QEILGCPVQV ISGEEEARLI 130 140 150 160 170 180 YQGVAHTTGG ADQRLVVDIS GASTELVTGT GAQTTSLFSL SMGCVTWLER YFADRNLGQE 190 200 210 220 230 240 NFDAAEKAAR EVLRPVADEL RYHGWKVCVG ASGTVQALQE IMMAQGMDER ITLEKLQQLK 250 260 270 280 290 300 QRAIHCGRLE ELEIDGLTLE RALVFPSGLA ILIAIFTELN IQCMTLAGGA LREGLVYGML 310 320 330 340 350 360 HLAVEQDIRS RTLRNIQRRF MIDIDQAQRV AKVAANFFDQ VENEWHLEAI SRDLLISACQ 370 380 390 400 410 420 LHEIGLSVDF KQAPQHAAYL VRNLDLPGFT PAQKKLLATL LLNQTNPVDL SSLHQQNAVP 430 440 450 460 470 480 PRVAEQLCRL LRLAIIFASR RRDDLVPEMT LQANHELLTL TLPQGWLTQH PLGKEIIAQE 490 NQWQSYVHWP LEVH

References

[1]

"Complete genome sequence of Shigella flexneri 5b and comparison with Shigella flexneri 2a." Nie H., Yang F., Zhang X., Yang J., Chen L., Wang J., Xiong Z., Peng J., Sun L., Dong J., Xue Y., Xu X., Chen S., Yao Z., Shen Y., Jin Q. BMC Genomics 7:173-173(2006) [PubMed: 16822325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 8401.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000266 Genomic DNA. Translation: ABF05746.1.
RefSeq	YP_691051.1. NC_008258.1.
3D structure databases
ProteinModelPortal	Q0SYW9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SYW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000096244; EBESCP00000092440; EBESCG00000095288.
GeneID	4211124.
GenomeReviews	Gene locus SFV_3725 in contig CP000266_GR.
KEGG	sfv:SFV_3725.
PATRIC	18731591. VBIShiFle33408_4242.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0248.
GeneTree	EBGT00050000009690.
HOGENOM	HBG451789.
OMA	MQRGWQC.
ProtClustDB	PRK11031.
Enzyme and pathway databases
BioCyc	SFLE373384:SFV_3725-MONOMER.
Family and domain databases
HAMAP	MF_01550. GppA. [Tree]
InterPro	IPR023709. Guo-5TP_3DP_PyrP. IPR003695. Ppx_GppA. [Graphical view]
KO	K01524.
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPPA_SHIF8

Accession

Primary (citable) accession number: Q0SYW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program