Reviewed, UniProtKB/Swiss-Prot Q0SV31 (AROE_CLOPS)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	CPR_0693

Organism

Clostridium perfringens (strain SM101 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

289380 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP(+) = 3-dehydroshikimate + NADPH.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7.
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Shikimate dehydrogenase		PRO_1000021278
Regions
	Nucleotide binding	125 – 129	5	NADP By similarity
Sites
	Active site	65	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0SV31-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 940043DF69B7C38B	FASTA	271	30,555
	10 20 30 40 50 60 MKLFGLIGEK LGHSLSPEIH NKVFKDNNID GLYNLFSVKK DFENNIVESL KCLGVRGANV 70 80 90 100 110 120 TIPYKEKVMN QLDIISHEAK AIGAVNTILI KDGKSYGYNT DYYGFGKMLE RAKVDIEGNS 130 140 150 160 170 180 FFVLGAGGAA RSILKYLEDS KAKKIVLVSR DKEKVFKKFK DFNINFMSYG ELEEIHEEFA 190 200 210 220 230 240 LINTTPCGMY PNTNSVAVSE KVIKKFKVAL DIVYNPLETK FLKMAKDNGL KTVDGLFMLV 250 260 270 GQGVKAEEIW NGIKVDKSTE ENIYEELKCR F

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000312 Genomic DNA. Translation: ABG85376.1.
RefSeq	YP_698019.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4204010.
GenomeReviews	Gene locus CPR_0693 in contig CP000312_GR.
KEGG	cpr:CPR_0693.
TIGR	CPR_0693.
Phylogenomic databases
HOGENOM	Q0SV31.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR016040. NAD(P)-bd. IPR011342. Quinate/shikimate_5-DHase. IPR013708. Shikimate_DHase-bd_N. IPR006151. Shikm_DHase/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_CLOPS

Accession

Primary (citable) accession number: Q0SV31

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)