Q0SU18 (SYE_CLOPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	CPR_1067

Organism

Clostridium perfringens (strain SM101 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

289380 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000367651
Regions
	Motif	41 – 51	11	"HIGH" region HAMAP MF_00022_B
	Motif	293 – 297	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	296	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SU18 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: A8EA61A0C59C4F58	FASTA	552	63,760
	10 20 30 40 50 60 MSYENLANLI FPNIDKTPEY YFEKYPKRDL KEGAKVLRYA PSPTGFQHIG GVFASLINER 70 80 90 100 110 120 LAHQSGGIFY LRIEDTDQKR EVEGAIDDTI KTMHNFGMDF DEGITGENSE KGAYAPYKQS 130 140 150 160 170 180 QRADIYRAFV KDLLRKGLAY PCFMTSEELE ALREKQIAEK LTPGCYGEFA KYRDLSPEEA 190 200 210 220 230 240 IKRIEAGENY VIRMKSPGNP EKRVVAHDMI KGEVSFPENL QDVVIIKGDG LPTYHFAHAI 250 260 270 280 290 300 DDTLMRTTHV IRGEEWLSSL PIHLQMFEVL GVEAPKYAHI PTIMKMDGSS KRKLSKRKDP 310 320 330 340 350 360 ESAVSYYSEK GYPSQSVIEY LLNIINSAFE EWRAENPDAD YHDYKVELDK MSKSGALFDL 370 380 390 400 410 420 VKLNDVSKDV ICKMKPEVVY DLYTNWAKEY DKEMYDLVTS KEAMMKEVFN IDKEGPKPRK 430 440 450 460 470 480 DFAKWDEVRE KIFYFFDELF DKETANDVEL PKTLELEEAK RIIEAYEKAY NFNTDKDTWF 490 500 510 520 530 540 SDLKEVAVEL GYATDRKKYK KNPEEYKGMV SDVAGAVRAA LTHRANTPDL YTIMQIMGEE 550 AVRERFKKFL AL

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SM101 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000312 Genomic DNA. Translation: ABG86873.1.
RefSeq	YP_698389.1. NC_008262.1.
3D structure databases
ProteinModelPortal	Q0SU18.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SU18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4204243.
GenomeReviews	Gene locus CPR_1067 in contig CP000312_GR.
KEGG	cpr:CPR_1067.
PATRIC	19490425. VBICloPer122123_1043.
TIGR	CPR_1067.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DKETAND.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	CPER289380:CPR_1067-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CLOPS

Accession

Primary (citable) accession number: Q0SU18

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program