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Q0SRS5

- DAPA_CLOPS

UniProt

Q0SRS5 - DAPA_CLOPS

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Protein
4-hydroxy-tetrahydrodipicolinate synthase
Gene
dapA, CPR_1872
Organism
Clostridium perfringens (strain SM101 / Type A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA) By similarity.UniRule annotation

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei43 – 431Part of a proton relay during catalysis By similarity
Binding sitei44 – 441Pyruvate By similarity
Sitei106 – 1061Part of a proton relay during catalysis By similarity
Active sitei132 – 1321Proton donor/acceptor By similarity
Active sitei160 – 1601Schiff-base intermediate with substrate By similarity
Binding sitei202 – 2021Pyruvate; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
  2. amine-lyase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciCPER289380:GI76-1883-MONOMER.
UniPathwayiUPA00034; UER00017.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthase (EC:4.3.3.7)
Short name:
HTPA synthase
Gene namesi
Name:dapA
Ordered Locus Names:CPR_1872
OrganismiClostridium perfringens (strain SM101 / Type A)
Taxonomic identifieri289380 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000001824: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2912914-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation
PRO_1000050180Add
BLAST

Interactioni

Subunit structurei

Homotetramer; dimer of dimers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi289380.CPR_1872.

Structurei

3D structure databases

ProteinModelPortaliQ0SRS5.

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173604.
KOiK01714.
OMAiGNEMATM.
OrthoDBiEOG6W7235.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00418. DapA.
InterProiIPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
TIGRFAMsiTIGR00674. dapA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0SRS5-1 [UniParc]FASTAAdd to Basket

« Hide

MFKGSCVALI TPFTEDGVNY EELRKLLEWH IKNHTDAILV CGTTGEGSTM    50
TLEEKKEVIK FSVEVVNKRV PVIAGTGTNN TKASIELSKY AEEVGADMVL 100
IITPYYNKTS QKGLYAHFSA INDAINIPIM LYNVPSRTGM NITPLMLDKL 150
ANLNNVVAIK EASGDLSQVA KMAELCGDRI AIYSGNDDQI VPILSLGGAG 200
VVSVLANILP EETHNICEKY FLGEVIESRN LQLKYLSLAN SLFIETNPIP 250
VKTAMNLMNF NCGPLRLPLC EMEDSNLVIL EENLKANGLI K 291
Length:291
Mass (Da):31,897
Last modified:September 5, 2006 - v1
Checksum:iC944090AA251C912
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000312 Genomic DNA. Translation: ABG86666.1.
RefSeqiYP_699182.1. NC_008262.1.

Genome annotation databases

EnsemblBacteriaiABG86666; ABG86666; CPR_1872.
GeneIDi4205617.
KEGGicpr:CPR_1872.
PATRICi19492049. VBICloPer122123_1848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000312 Genomic DNA. Translation: ABG86666.1 .
RefSeqi YP_699182.1. NC_008262.1.

3D structure databases

ProteinModelPortali Q0SRS5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 289380.CPR_1872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG86666 ; ABG86666 ; CPR_1872 .
GeneIDi 4205617.
KEGGi cpr:CPR_1872.
PATRICi 19492049. VBICloPer122123_1848.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000173604.
KOi K01714.
OMAi GNEMATM.
OrthoDBi EOG6W7235.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00017 .
BioCyci CPER289380:GI76-1883-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00418. DapA.
InterProi IPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
TIGRFAMsi TIGR00674. dapA. 1 hit.
PROSITEi PS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SM101 / Type A.

Entry informationi

Entry nameiDAPA_CLOPS
AccessioniPrimary (citable) accession number: Q0SRS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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