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Q0SRS5

- DAPA_CLOPS

UniProt

Q0SRS5 - DAPA_CLOPS

Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA

Organism
Clostridium perfringens (strain SM101 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotation

    Catalytic activityi

    Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei43 – 431Part of a proton relay during catalysisUniRule annotation
    Binding sitei44 – 441PyruvateUniRule annotation
    Sitei106 – 1061Part of a proton relay during catalysisUniRule annotation
    Active sitei132 – 1321Proton donor/acceptorUniRule annotation
    Active sitei160 – 1601Schiff-base intermediate with substrateUniRule annotation
    Binding sitei202 – 2021Pyruvate; via carbonyl oxygenUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
    2. amine-lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciCPER289380:GI76-1883-MONOMER.
    UniPathwayiUPA00034; UER00017.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation (EC:4.3.3.7UniRule annotation)
    Short name:
    HTPA synthaseUniRule annotation
    Gene namesi
    Name:dapAUniRule annotation
    Ordered Locus Names:CPR_1872
    OrganismiClostridium perfringens (strain SM101 / Type A)
    Taxonomic identifieri289380 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000001824: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2912914-hydroxy-tetrahydrodipicolinate synthasePRO_1000050180Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi289380.CPR_1872.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0SRS5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DapA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0329.
    HOGENOMiHOG000173604.
    KOiK01714.
    OMAiGNEMATM.
    OrthoDBiEOG6W7235.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00418. DapA.
    InterProiIPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    TIGRFAMsiTIGR00674. dapA. 1 hit.
    PROSITEiPS00665. DHDPS_1. 1 hit.
    PS00666. DHDPS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0SRS5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKGSCVALI TPFTEDGVNY EELRKLLEWH IKNHTDAILV CGTTGEGSTM    50
    TLEEKKEVIK FSVEVVNKRV PVIAGTGTNN TKASIELSKY AEEVGADMVL 100
    IITPYYNKTS QKGLYAHFSA INDAINIPIM LYNVPSRTGM NITPLMLDKL 150
    ANLNNVVAIK EASGDLSQVA KMAELCGDRI AIYSGNDDQI VPILSLGGAG 200
    VVSVLANILP EETHNICEKY FLGEVIESRN LQLKYLSLAN SLFIETNPIP 250
    VKTAMNLMNF NCGPLRLPLC EMEDSNLVIL EENLKANGLI K 291
    Length:291
    Mass (Da):31,897
    Last modified:September 5, 2006 - v1
    Checksum:iC944090AA251C912
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000312 Genomic DNA. Translation: ABG86666.1.
    RefSeqiYP_699182.1. NC_008262.1.

    Genome annotation databases

    EnsemblBacteriaiABG86666; ABG86666; CPR_1872.
    GeneIDi4205617.
    KEGGicpr:CPR_1872.
    PATRICi19492049. VBICloPer122123_1848.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000312 Genomic DNA. Translation: ABG86666.1 .
    RefSeqi YP_699182.1. NC_008262.1.

    3D structure databases

    ProteinModelPortali Q0SRS5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 289380.CPR_1872.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG86666 ; ABG86666 ; CPR_1872 .
    GeneIDi 4205617.
    KEGGi cpr:CPR_1872.
    PATRICi 19492049. VBICloPer122123_1848.

    Phylogenomic databases

    eggNOGi COG0329.
    HOGENOMi HOG000173604.
    KOi K01714.
    OMAi GNEMATM.
    OrthoDBi EOG6W7235.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00017 .
    BioCyci CPER289380:GI76-1883-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00418. DapA.
    InterProi IPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    TIGRFAMsi TIGR00674. dapA. 1 hit.
    PROSITEi PS00665. DHDPS_1. 1 hit.
    PS00666. DHDPS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SM101 / Type A.

    Entry informationi

    Entry nameiDAPA_CLOPS
    AccessioniPrimary (citable) accession number: Q0SRS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.Curated

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3