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Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA

Organism
Clostridium perfringens (strain SM101 / Type A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotation

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.Curated

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 3 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (CPR_1650)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei43Part of a proton relay during catalysisUniRule annotation1
Binding sitei44PyruvateUniRule annotation1
Sitei106Part of a proton relay during catalysisUniRule annotation1
Active sitei132Proton donor/acceptorUniRule annotation1
Active sitei160Schiff-base intermediate with substrateUniRule annotation1
Binding sitei202Pyruvate; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00034; UER00017

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation (EC:4.3.3.7UniRule annotation)
Short name:
HTPA synthaseUniRule annotation
Gene namesi
Name:dapAUniRule annotation
Ordered Locus Names:CPR_1872
OrganismiClostridium perfringens (strain SM101 / Type A)
Taxonomic identifieri289380 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000001824 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000501801 – 2914-hydroxy-tetrahydrodipicolinate synthaseAdd BLAST291

Proteomic databases

PRIDEiQ0SRS5

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ0SRS5
SMRiQ0SRS5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000173604
KOiK01714
OMAiMSWDEHV

Family and domain databases

CDDicd00950 DHDPS, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00418 DapA, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR005263 DapA
IPR002220 DapA-like
IPR020625 Schiff_base-form_aldolases_AS
IPR020624 Schiff_base-form_aldolases_CS
PANTHERiPTHR12128 PTHR12128, 1 hit
PfamiView protein in Pfam
PF00701 DHDPS, 1 hit
PIRSFiPIRSF001365 DHDPS, 1 hit
PRINTSiPR00146 DHPICSNTHASE
SMARTiView protein in SMART
SM01130 DHDPS, 1 hit
TIGRFAMsiTIGR00674 dapA, 1 hit
PROSITEiView protein in PROSITE
PS00665 DHDPS_1, 1 hit
PS00666 DHDPS_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q0SRS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKGSCVALI TPFTEDGVNY EELRKLLEWH IKNHTDAILV CGTTGEGSTM
60 70 80 90 100
TLEEKKEVIK FSVEVVNKRV PVIAGTGTNN TKASIELSKY AEEVGADMVL
110 120 130 140 150
IITPYYNKTS QKGLYAHFSA INDAINIPIM LYNVPSRTGM NITPLMLDKL
160 170 180 190 200
ANLNNVVAIK EASGDLSQVA KMAELCGDRI AIYSGNDDQI VPILSLGGAG
210 220 230 240 250
VVSVLANILP EETHNICEKY FLGEVIESRN LQLKYLSLAN SLFIETNPIP
260 270 280 290
VKTAMNLMNF NCGPLRLPLC EMEDSNLVIL EENLKANGLI K
Length:291
Mass (Da):31,897
Last modified:September 5, 2006 - v1
Checksum:iC944090AA251C912
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000312 Genomic DNA Translation: ABG86666.1

Genome annotation databases

EnsemblBacteriaiABG86666; ABG86666; CPR_1872
KEGGicpr:CPR_1872

Similar proteinsi

Entry informationi

Entry nameiDAPA_CLOPS
AccessioniPrimary (citable) accession number: Q0SRS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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