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Q0SRQ2 (GLYA_CLOPS) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
Short name=SHMT
Short name=Serine methylase
EC=2.1.2.1
Gene names
Name:glyA
Ordered Locus Names:CPR_1895
OrganismClostridium perfringens (strain SM101 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier289380 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_00051

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00051.

Sequence similarities

Belongs to the SHMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410Pyridoxal-phosphate-dependent serine hydroxymethyltransferase
PRO_1000006241

Regions

Region123 – 1253Substrate binding By similarity

Sites

Binding site331Pyridoxal phosphate By similarity
Binding site531Pyridoxal phosphate By similarity
Binding site551Substrate By similarity
Binding site621Substrate binding By similarity
Binding site631Pyridoxal phosphate By similarity
Binding site971Pyridoxal phosphate By similarity
Binding site1191Substrate By similarity
Binding site1741Pyridoxal phosphate By similarity
Binding site2021Pyridoxal phosphate By similarity
Binding site2271Pyridoxal phosphate By similarity
Binding site2341Pyridoxal phosphate By similarity
Binding site2591Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site3591Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2281N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0SRQ2 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 2CB29FB1A86B3F19

FASTA41045,205
        10         20         30         40         50         60 
MNFDNLERED EQIAHLVQKE KERQENSIEL IASENFVSKA VMEAMGSYLT NKYAEGYPSK 

        70         80         90        100        110        120 
RYYGGCHVVD EVEDLARERV KKLFGAEHAN VQPHSGSQAN MAVYFSILES GDTVLGMDLS 

       130        140        150        160        170        180 
HGGHLTHGSP VNFSGRLFNF VSYGVDKETE TINYETVREL ALKHKPKLIV AGASAYSRII 

       190        200        210        220        230        240 
DFKTLREIAD EVGAYLMVDI AHIAGLVATG LHPSPVPYAD FVTSTTHKTL RGPRGGLILC 

       250        260        270        280        290        300 
KEKFAKVLDK NIFPGIQGGP LMHIIAAKAV CFKEALEPSF KTYMEQVVKN AHVLAEALES 

       310        320        330        340        350        360 
YGFKLVSNGT DNHLILVDLT NKDITGKDAE ILLDSIGITL NKNTVPNETR SPFVTSGVRI 

       370        380        390        400        410 
GTPAITTRGF KEEEMKEIAS IINDAIKEKD GDLEPLKARV KALCAKYPLY

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000312 Genomic DNA. Translation: ABG85878.1.
RefSeqYP_699205.1. NC_008262.1.

3D structure databases

ProteinModelPortalQ0SRQ2.
SMRQ0SRQ2. Positions 6-403.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0SRQ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4205095.
GenomeReviewsGene locus CPR_1895 in contig CP000312_GR.
KEGGcpr:CPR_1895.
PATRIC19492095. VBICloPer122123_1871.
TIGRCPR_1895.

Phylogenomic databases

eggNOGCOG0112.
HOGENOMHBG301263.
OMAGTSNHLM.
ProtClustDBPRK00011.

Enzyme and pathway databases

BioCycCPER289380:CPR_1895-MONOMER.

Family and domain databases

HAMAPMF_00051. SHMT.
[Tree]
InterProIPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00600.
PANTHERPTHR11680. Gly_HO-Metrfase. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLYA_CLOPS
AccessionPrimary (citable) accession number: Q0SRQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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