ID   TGT_CLOPS               Reviewed;         380 AA.
AC   Q0SRN5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=Queuine tRNA-ribosyltransferase;
DE            EC=2.4.2.29;
DE   AltName: Full=tRNA-guanine transglycosylase;
DE   AltName: Full=Guanine insertion enzyme;
GN   Name=tgt; OrderedLocusNames=CPR_1912;
OS   Clostridium perfringens (strain SM101 / Type A).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=289380;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16825665; DOI=10.1101/gr.5238106;
RA   Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R.,
RA   DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C.,
RA   Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J.,
RA   Sullivan S.A., Khouri H., Dimitrov G.I., Watkins K.L., Mulligan S.,
RA   Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H.,
RA   Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I.,
RA   Melville S.B., Paulsen I.T.;
RT   "Skewed genomic variability in strains of the toxigenic bacterial
RT   pathogen, Clostridium perfringens.";
RL   Genome Res. 16:1031-1040(2006).
CC   -!- FUNCTION: Exchanges the guanine residue with 7-aminomethyl-7-
CC       deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His
CC       and -Tyr). After this exchange, a cyclopentendiol moiety is
CC       attached to the 7-aminomethyl group of 7-deazaguanine, resulting
CC       in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-
CC       dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: [tRNA]-guanine + queuine = [tRNA]-queuine +
CC       guanine.
CC   -!- CATALYTIC ACTIVITY: [tRNA]-guanine + 7-aminomethyl-7-carbaguanine
CC       = [tRNA]-7-aminomethyl-7-carbaguanine + guanine.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: tRNA modification; queuosine-tRNA biosynthesis.
CC   -!- SIMILARITY: Belongs to the queuine tRNA-ribosyltransferase family.
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DR   EMBL; CP000312; ABG87624.1; -; Genomic_DNA.
DR   RefSeq; YP_699222.1; -.
DR   GeneID; 4206159; -.
DR   GenomeReviews; CP000312_GR; CPR_1912.
DR   KEGG; cpr:CPR_1912; -.
DR   TIGR; CPR_1912; -.
DR   HOGENOM; Q0SRN5; -.
DR   OMA; Q0SRN5; VLNSDIV.
DR   GO; GO:0008479; F:queuine tRNA-ribosyltransferase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00168; -; 1.
DR   InterPro; IPR004803; QtRNA_ribo_trans.
DR   InterPro; IPR002616; tRNA_ribo_trans.
DR   Gene3D; G3DSA:3.20.20.105; tRNA_ribo_trans; 1.
DR   PANTHER; PTHR11962; tRNA_ribo_trans; 1.
DR   Pfam; PF01702; TGT; 1.
DR   TIGRFAMs; TIGR00430; Q_tRNA_tgt; 1.
DR   TIGRFAMs; TIGR00449; tgt_general; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Glycosyltransferase; Metal-binding;
KW   Queuosine biosynthesis; Transferase; tRNA processing; Zinc.
FT   CHAIN         1    380       Queuine tRNA-ribosyltransferase.
FT                                /FTId=PRO_1000016783.
FT   ACT_SITE     93     93       Nucleophile (By similarity).
FT   METAL       313    313       Zinc (By similarity).
FT   METAL       315    315       Zinc (By similarity).
FT   METAL       318    318       Zinc (By similarity).
FT   METAL       344    344       Zinc (By similarity).
FT   BINDING      94     94       Substrate (By similarity).
SQ   SEQUENCE   380 AA;  43186 MW;  113B6B73540539D6 CRC64;
     MTKKRYTLLK KDGKARRGEF VTPHGTIQTP VFMNVGTLAA IKGAVSSMDL KEIGCQVELS
     NTYHLHLRPG DKIVKQMGGL HNFMNWDRPI LTDSGGFQVF SLAGMRKIKE EGVYFNSHID
     GRKIFMGPEE SMQIQSNLGS TIAMAFDECI PNPSTREYVE KSVARTTRWL ERCKKEMDRL
     NSLDDTVNKE QMLFGINQGG VYEDIRIEHA KTIREMDLDG YAIGGLAVGE THEEMYRVID
     AVVPHLPEDK PIYLMGVGLP SNILEAVERG VDFFDCVLPA RNGRHGHVFT KEGKINLMNA
     KFELDARPID EGCQCPACKN YTRAYIRHLF KAKEMLAMRL CVLHNLYFYN KLMEDIRDAI
     DGGYFAEFKA KKLEEWNGRA
//