Reviewed, UniProtKB/Swiss-Prot Q0SRN5 (TGT_CLOPS)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	CPR_1912

Organism

Clostridium perfringens (strain SM101 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

289380 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_1000016783
Sites
	Active site	93	1	Nucleophile By similarity
	Metal binding	313	1	Zinc By similarity
	Metal binding	315	1	Zinc By similarity
	Metal binding	318	1	Zinc By similarity
	Metal binding	344	1	Zinc By similarity
	Binding site	94	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SRN5-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 113B6B73540539D6	FASTA	380	43,186
	10 20 30 40 50 60 MTKKRYTLLK KDGKARRGEF VTPHGTIQTP VFMNVGTLAA IKGAVSSMDL KEIGCQVELS 70 80 90 100 110 120 NTYHLHLRPG DKIVKQMGGL HNFMNWDRPI LTDSGGFQVF SLAGMRKIKE EGVYFNSHID 130 140 150 160 170 180 GRKIFMGPEE SMQIQSNLGS TIAMAFDECI PNPSTREYVE KSVARTTRWL ERCKKEMDRL 190 200 210 220 230 240 NSLDDTVNKE QMLFGINQGG VYEDIRIEHA KTIREMDLDG YAIGGLAVGE THEEMYRVID 250 260 270 280 290 300 AVVPHLPEDK PIYLMGVGLP SNILEAVERG VDFFDCVLPA RNGRHGHVFT KEGKINLMNA 310 320 330 340 350 360 KFELDARPID EGCQCPACKN YTRAYIRHLF KAKEMLAMRL CVLHNLYFYN KLMEDIRDAI 370 380 DGGYFAEFKA KKLEEWNGRA

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000312 Genomic DNA. Translation: ABG87624.1.
RefSeq	YP_699222.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4206159.
GenomeReviews	Gene locus CPR_1912 in contig CP000312_GR.
KEGG	cpr:CPR_1912.
TIGR	CPR_1912.
Phylogenomic databases
HOGENOM	Q0SRN5.
OMA	Q0SRN5. VLNSDIV.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_CLOPS

Accession

Primary (citable) accession number: Q0SRN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)