Q0SRD5 (GPR_CLOPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Germination protease
EC=3.4.24.78

Alternative name(s):
GPR endopeptidase
Germination proteinase
Spore protease

Gene names

Name:	gpr
Ordered Locus Names:	CPR_2013

Organism

Clostridium perfringens (strain SM101 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

289380 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Initiates the rapid degradation of small, acid-soluble proteins during spore germination By similarity. HAMAP MF_00626
Catalytic activity	Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala. HAMAP MF_00626
Subunit structure	Homotetramer By similarity. HAMAP MF_00626
Post-translational modification	Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination By similarity. HAMAP MF_00626
Sequence similarities	Belongs to the peptidase A25 family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW spore germination Inferred from electronic annotation. Source: InterPro
Molecular function	peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 7	7	By similarity		PRO_0000316072
	Chain	8 – 325	318	Germination protease HAMAP MF_00626		PRO_1000051611

Sequences

	Sequence		Length	Mass (Da)
	Q0SRD5 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: AFE78A082787EFBF	FASTA	325	35,620
	10 20 30 40 50 60 MYNVRTDLAV ESREIYKHRY NREIDGVVFE EKTVEEDIKV TNVDILNEEG AKAMEKPIGR 70 80 90 100 110 120 YVTIDIPDYT HYDGGIMDEV SHVVAASLEE LINLPEERTA LVVGLGNWNV TPDAIGPKVV 130 140 150 160 170 180 GKLMVTRHLK KVMPDIIDDS VRPVCAIAPG VLGITGIETG EIIKSLVEKI KPDLVVCIDA 190 200 210 220 230 240 LASRKLERVA RTIQISNTGI SPGAGVGNHR MQINEESLGI PVIALGVPTV VDAATIANDA 250 260 270 280 290 300 MDLVLDEMIN QADAGKEFYN ILNNIDKNEK GMMIKSLLDP YVGDLMVTPK EIDDIIESVS 310 320 KIIANGINIA LQPNMVLEDI NKFLN

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SM101 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000312 Genomic DNA. Translation: ABG87065.1.
RefSeq	YP_699322.1. NC_008262.1.
3D structure databases
ProteinModelPortal	Q0SRD5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SRD5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4205316.
GenomeReviews	Gene locus CPR_2013 in contig CP000312_GR.
KEGG	cpr:CPR_2013.
PATRIC	19492331. VBICloPer122123_1988.
TIGR	CPR_2013.
Phylogenomic databases
eggNOG	NOG04272.
HOGENOM	HBG304865.
OMA	VNTTIQI.
ProtClustDB	PRK12362.
Enzyme and pathway databases
BioCyc	CPER289380:CPR_2013-MONOMER.
Family and domain databases
HAMAP	MF_00626. Germination_prot. [Tree]
InterPro	IPR023430. Pept_HybD-like_dom. IPR005080. Peptidase_A25. [Graphical view]
Gene3D	G3DSA:3.40.50.1450. Peptidase_A31. 1 hit.
KO	K06012.
Pfam	PF03418. Peptidase_A25. 2 hits. [Graphical view]
PIRSF	PIRSF019549. Peptidase_A25. 1 hit.
ProDom	PD041835. Peptidase_A25. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF53163. SSF53163. 1 hit.
TIGRFAMs	TIGR01441. GPR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPR_CLOPS

Accession

Primary (citable) accession number: Q0SRD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program