Q0SQU1 (RNPH_CLOPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	CPR_2240

Organism

Clostridium perfringens (strain SM101 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

289380 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Ribonuclease PH HAMAP MF_00564		PRO_1000024797

Sequences

	Sequence		Length	Mass (Da)
	Q0SQU1 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: 9F5BB6A32DA93CC1	FASTA	248	27,682
	10 20 30 40 50 60 MRSSGRKKEQ IRPVKITRNF TKYAEGSVLI EVGDTKVLCT ASIEEKVPPF LKGSGEGWIT 70 80 90 100 110 120 AEYNMIPRST QSRKQRDINK LKIDGRTMEI QRLIGRALRS AVDMKALGEK TIWIDCDVLQ 130 140 150 160 170 180 ADGGTRTTSI TGSFVALVDA VNKLHQKKPF NVYPIRHFVS AVSIGIVGEE KVLDLCYEED 190 200 210 220 230 240 HVAKVDMNVV MIEEGEFIEI QGTGEIGPFS RKELDELLNL AEKGAKQMIQ AQKNALKTDS LWIGTGRE

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SM101 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000312 Genomic DNA. Translation: ABG85418.1.
RefSeq	YP_699516.1. NC_008262.1.
3D structure databases
ProteinModelPortal	Q0SQU1.
SMR	Q0SQU1. Positions 2-238.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SQU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4204122.
GenomeReviews	Gene locus CPR_2240 in contig CP000312_GR.
KEGG	cpr:CPR_2240.
PATRIC	19492787. VBICloPer122123_2181.
TIGR	CPR_2240.
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	CPER289380:CPR_2240-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_CLOPS

Accession

Primary (citable) accession number: Q0SQU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 19, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program