Q0SQE1 (EFG_CLOPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	CPR_2403

Organism

Clostridium perfringens (strain SM101 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

289380 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Elongation factor G HAMAP MF_00054_B		PRO_0000263443
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SQE1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: A2DB9AF65370ABF9	FASTA	688	75,987
	10 20 30 40 50 60 MARQYPLEKF RNFGIMAHID AGKTTTTERI LFYTGRNHKI GETHDGASTM DWMAQEQERG 70 80 90 100 110 120 ITITSAATTC FWKGYELNII DTPGHVDFTV EVERSLRVLD GAVTVLDAKS GVEPQTETVW 130 140 150 160 170 180 RQADKYGVPR MIYVNKMDAT GADYYNCINT VRERLQANAV AIQIPIGQED QFQGMVDLLT 190 200 210 220 230 240 NQAIIFKDDL GKDIEVGEVP ADLADKAEEY RAAMIEAIAE TDEELMMKYL EGEELTLEEL 250 260 270 280 290 300 KVALRKATIN NEIIPVICGS SYKNKGVQQM IDGVVDYLPS PLDIPAVKGT NLDGEEEVRE 310 320 330 340 350 360 ASDDAPMSAL AFKIATDPFV GRLAFTRVYS GVLESGSYVL NSTKGKKERI GRLVKMHANS 370 380 390 400 410 420 REEVESLEAA ELGAVIGLKN TTTGDTLCTE AAPIILEKME FPEPVISIAI EPKTKAGQEK 430 440 450 460 470 480 MGIALSKLAE EDPTFKTWTD QETGQTIIAG MGELHLDIIV DRLQREFKVE CNVGAPQVAY 490 500 510 520 530 540 KETIKKAVEA EAKFARQSGG RGQYGHCKIE MIPTEGEYEF ENAIVGGAIP REYIPAVDNG 550 560 570 580 590 600 IREAAESGII AGYPVINFKI RLFDGSYHDV DSSEMAFKIA GSMAFKNAMA KADAVLLEPI 610 620 630 640 650 660 MKVEITVPEE YMGDVIGDVN SRRGRMEGMD SRNGAQIIRA FIPLSEMFGY ATALRSRTQG 670 680 RGTYAMEFDH YDDVPKSIQE EVAGKKNK

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SM101 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000312 Genomic DNA. Translation: ABG85516.1.
RefSeq	YP_699666.1. NC_008262.1.
3D structure databases
ProteinModelPortal	Q0SQE1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SQE1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4204062.
GenomeReviews	Gene locus CPR_2403 in contig CP000312_GR.
KEGG	cpr:CPR_2403.
PATRIC	19493116. VBICloPer122123_2336.
TIGR	CPR_2403.
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK00007.
Enzyme and pathway databases
BioCyc	CPER289380:CPR_2403-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_CLOPS

Accession

Primary (citable) accession number: Q0SQE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program