Q0SQ45 (ADD_CLOPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:	add
Ordered Locus Names:	CPR_2515

Organism

Clostridium perfringens (strain SM101 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

289380 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Adenosine + H2O = inosine + NH3. HAMAP MF_00540
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00540
Sequence similarities	Belongs to the adenosine and AMP deaminases family. Adenosine deaminase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosine deaminase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Adenosine deaminase HAMAP MF_00540		PRO_1000017658
Sites
	Active site	200	1	Proton donor By similarity
	Metal binding	12	1	Zinc; catalytic By similarity
	Metal binding	14	1	Zinc; catalytic By similarity
	Metal binding	197	1	Zinc; catalytic By similarity
	Metal binding	278	1	Zinc; catalytic By similarity
	Binding site	14	1	Substrate By similarity
	Binding site	16	1	Substrate By similarity
	Binding site	170	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Site	221	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SQ45 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 0AD78DDAEA6D6CF3	FASTA	332	38,086
	10 20 30 40 50 60 MNLLNLPKIE LHCHLDGSLR VETAIELAKK EGIKLHSYEY DKVKELLVIS KECNSLEDYL 70 80 90 100 110 120 NRFALPAKLL QRPENLERVA FELMEDASKE NVKYIEIRFA PLLHLEKGMT QKEVIESVIK 130 140 150 160 170 180 GIRKAEEFYD IKGNLILSCL RHHSIDSVYE VIEEGKNFIG KGVVAIDLAG GELEGFVKPY 190 200 210 220 230 240 KEVMKLARES GFRVTIHAGE TGYGKNVRDA IELLGAERIG HGLFIFNDEE AYNLVKEKGV 250 260 270 280 290 300 TLEMCPKSNI DTKGVNKYEE HPIYKYHKDN IRVNLSTDNR TVSNINLTEE FENVHKTFNI 310 320 330 DFEDYKKIYL NSVEASFCSE ELKEKLKLSI II

References

[1]

"Skewed genomic variability in strains of the toxigenic bacterial pathogen, Clostridium perfringens." Myers G.S.A., Rasko D.A., Cheung J.K., Ravel J., Seshadri R., DeBoy R.T., Ren Q., Varga J., Awad M.M., Brinkac L.M., Daugherty S.C., Haft D.H., Dodson R.J., Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Khouri H. , Dimitrov G.I., Watkins K.L., Mulligan S., Benton J., Radune D., Fisher D.J., Atkins H.S., Hiscox T., Jost B.H., Billington S.J., Songer J.G., McClane B.A., Titball R.W., Rood J.I., Melville S.B., Paulsen I.T. Genome Res. 16:1031-1040(2006) [PubMed: 16825665] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SM101 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000312 Genomic DNA. Translation: ABG85954.1.
RefSeq	YP_699762.1. NC_008262.1.
3D structure databases
ProteinModelPortal	Q0SQ45.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SQ45.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4205270.
GenomeReviews	Gene locus CPR_2515 in contig CP000312_GR.
KEGG	cpr:CPR_2515.
PATRIC	19493350. VBICloPer122123_2435.
TIGR	CPR_2515.
Phylogenomic databases
eggNOG	COG1816.
HOGENOM	HBG630382.
OMA	CKKYQQQ.
ProtClustDB	PRK09358.
Enzyme and pathway databases
BioCyc	CPER289380:CPR_2515-MONOMER.
Family and domain databases
HAMAP	MF_00540. A_deaminase. [Tree]
InterPro	IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADD_CLOPS

Accession

Primary (citable) accession number: Q0SQ45

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program