ID   TRUA_BORAP              Reviewed;         246 AA.
AC   Q0SPF2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=tRNA pseudouridine synthase A;
DE            EC=5.4.99.12;
DE   AltName: Full=tRNA-uridine isomerase I;
DE   AltName: Full=tRNA pseudouridylate synthase I;
GN   Name=truA; OrderedLocusNames=BAPKO_0011;
OS   Borrelia afzelii (strain PKo).
OC   Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Borrelia;
OC   Borrelia burgdorferi group.
OX   NCBI_TaxID=390236;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16914037; DOI=10.1186/1471-2164-7-211;
RA   Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M.,
RA   Suehnel J., Wilske B., Platzer M.;
RT   "Comparative genome analysis: selection pressure on the Borrelia vls
RT   cassettes is essential for infectivity.";
RL   BMC Genomics 7:211-211(2006).
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: tRNA uridine = tRNA pseudouridine.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase truA
CC       family.
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DR   EMBL; CP000395; ABH01276.1; -; Genomic_DNA.
DR   RefSeq; YP_709452.1; -.
DR   GeneID; 4227177; -.
DR   GenomeReviews; CP000395_GR; BAPKO_0011.
DR   KEGG; baf:BAPKO_0011; -.
DR   NMPDR; fig|390236.5.peg.79; -.
DR   HOGENOM; Q0SPF2; -.
DR   OMA; Q0SPF2; RRYRYTI.
DR   BioCyc; BAFZ390236:BAPKO_0011-MON; -.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR   HAMAP; MF_00171; -; 1.
DR   InterPro; IPR001406; tRNA_psdUrid_synth.
DR   Gene3D; G3DSA:3.30.70.580; PseudoU_synth_1; 1.
DR   Gene3D; G3DSA:3.30.70.660; PseudoU_synth_1; 1.
DR   PANTHER; PTHR11142; PseudoU_synth_1; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isomerase; tRNA processing.
FT   CHAIN         1    246       tRNA pseudouridine synthase A.
FT                                /FTId=PRO_1000017044.
FT   ACT_SITE     52     52       Nucleophile (By similarity).
SQ   SEQUENCE   246 AA;  28595 MW;  F79BBA14EE95E678 CRC64;
     MKKILAEIAY DGSMYHGFQI QPTKPTIQGE IEKALMKINK KKIKIHSSGR TDKGVHAKRQ
     IITFDININI QLNNLKKALN AILLKPGIKI LKLKHVKNSF HPRFNAQKRK YSYCILNSDN
     YYPWESYQAY YVNKKLNINN LNQMAKILIG NHDFTTFSCI KDKSKSKFRH IHSAKFKKKG
     KYIIFEIIGS SFLWKMVRSI IGTMLDIEIK NESISTFETI LKSKNRNLAR TTAPANALFL
     DKVYYE
//