Q0SPF2 (TRUA_BORAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	BAPKO_0011

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP MF_00171
Subunit structure	Homodimer By similarity. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_1000017044
Sites
	Active site	52	1	Nucleophile By similarity
	Binding site	111	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SPF2 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: F79BBA14EE95E678	FASTA	246	28,595
	10 20 30 40 50 60 MKKILAEIAY DGSMYHGFQI QPTKPTIQGE IEKALMKINK KKIKIHSSGR TDKGVHAKRQ 70 80 90 100 110 120 IITFDININI QLNNLKKALN AILLKPGIKI LKLKHVKNSF HPRFNAQKRK YSYCILNSDN 130 140 150 160 170 180 YYPWESYQAY YVNKKLNINN LNQMAKILIG NHDFTTFSCI KDKSKSKFRH IHSAKFKKKG 190 200 210 220 230 240 KYIIFEIIGS SFLWKMVRSI IGTMLDIEIK NESISTFETI LKSKNRNLAR TTAPANALFL DKVYYE

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000395 Genomic DNA. Translation: ABH01276.1.
RefSeq	YP_709452.1. NC_008277.1.
3D structure databases
ProteinModelPortal	Q0SPF2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SPF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000004043; EBBORP00000003661; EBBORG00000004043.
GeneID	4227177.
GenomeReviews	Gene locus BAPKO_0011 in contig CP000395_GR.
KEGG	baf:BAPKO_0011.
NMPDR	fig|390236.5.peg.79.
PATRIC	37188323. VBIBorAfz3878_0080.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
GeneTree	EBGT00050000007466.
HOGENOM	HBG621020.
OMA	QANAFVH.
ProtClustDB	PRK00021.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0011-MONOMER.
Family and domain databases
HAMAP	MF_00171. TruA. [Tree]
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
KO	K06173.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. HisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BORAP

Accession

Primary (citable) accession number: Q0SPF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program