Reviewed, UniProtKB/Swiss-Prot Q0SPF2 (TRUA_BORAP)

Names and origin

Protein names

Recommended name:
    tRNA pseudouridine synthase A
    EC=5.4.99.12
Alternative name(s):
    tRNA-uridine isomerase I
    tRNA pseudouridylate synthase I

Gene names

Name:	truA
Ordered Locus Names:	BAPKO_0011

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity.
Catalytic activity	tRNA uridine = tRNA pseudouridine. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase truA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_1000017044
Sites
	Active site	52	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SPF2-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: F79BBA14EE95E678	FASTA	246	28,595
	10 20 30 40 50 60 MKKILAEIAY DGSMYHGFQI QPTKPTIQGE IEKALMKINK KKIKIHSSGR TDKGVHAKRQ 70 80 90 100 110 120 IITFDININI QLNNLKKALN AILLKPGIKI LKLKHVKNSF HPRFNAQKRK YSYCILNSDN 130 140 150 160 170 180 YYPWESYQAY YVNKKLNINN LNQMAKILIG NHDFTTFSCI KDKSKSKFRH IHSAKFKKKG 190 200 210 220 230 240 KYIIFEIIGS SFLWKMVRSI IGTMLDIEIK NESISTFETI LKSKNRNLAR TTAPANALFL DKVYYE

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH01276.1.
RefSeq	YP_709452.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227177.
GenomeReviews	Gene locus BAPKO_0011 in contig CP000395_GR.
KEGG	baf:BAPKO_0011.
NMPDR	fig|390236.5.peg.79.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SPF2.
OMA	Q0SPF2. RRYRYTI.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0011-MON.
Family and domain databases
HAMAP	MF_00171. [Tree]
InterPro	IPR001406. tRNA_psdUrid_synth. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BORAP

Accession

Primary (citable) accession number: Q0SPF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)