Q0SPB0 (TPIS_BORAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	BAPKO_0055

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Triosephosphate isomerase HAMAP MF_00147_B		PRO_0000307438
Sites
	Active site	96	1	Electrophile By similarity
	Active site	169	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SPB0 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: EF737C12ACFCE32B	FASTA	253	27,830
	10 20 30 40 50 60 MRKTFLAGNW KMHYTSAEAS IVAKQIATEV KTLNDDFVIM ITPPFTALSK VSECIKGSNI 70 80 90 100 110 120 LLGAQNMSYM ESGARTSEIS PSMLLEFGVE YVILGHSECR LYLAETDEII NKKIRAGLKH 130 140 150 160 170 180 SFKYLILCVG ETLDERDSGK TLDVVLSQVK KGLDCVSESD IKRIILAYEP VWAIGTGKTA 190 200 210 220 230 240 TKEEAEEVHK AIRLEIMKLY SKSASDNIII QYGGSVNASN IKELMNEPNI DGALIGGASL 250 KAESFLSIIN NVR

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000395 Genomic DNA. Translation: ABH01318.1.
RefSeq	YP_709494.1. NC_008277.1.
3D structure databases
ProteinModelPortal	Q0SPB0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SPB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000004477; EBBORP00000004095; EBBORG00000004477.
GeneID	4227598.
GenomeReviews	Gene locus BAPKO_0055 in contig CP000395_GR.
KEGG	baf:BAPKO_0055.
NMPDR	fig|390236.5.peg.120.
PATRIC	37188407. VBIBorAfz3878_0121.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0149.
GeneTree	EBGT00050000007284.
HOGENOM	HBG708281.
OMA	HSERANE.
ProtClustDB	PRK00042.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0055-MONOMER.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_BORAP

Accession

Primary (citable) accession number: Q0SPB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program