Reviewed, UniProtKB/Swiss-Prot Q0SP63 (SYN_BORAP)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	BAPKO_0102

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000051382

Sequences

	Sequence		Length	Mass (Da)
	Q0SP63-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 6E71F055E9993DB7	FASTA	462	53,039
	10 20 30 40 50 60 MFASIKDILE NPILNSNVTI NGWIRTKRSN GKIGFIEIND GSTLKGIQAV INEEENQFDE 70 80 90 100 110 120 KDLKNLTTGA SISLTGLLVE SPAKGQNYEI KTCGFNVIGE ADPKTYPLQK KRHTFEFLRE 130 140 150 160 170 180 IPHLRIRTNT FGAVARVRSK ISYKIHEYFQ KNGFFYINTP IITSNDGEGA GEMFRVSTLK 190 200 210 220 230 240 FNKPNNDLGN IDFKDDFFGK EAFLSVTGQL HGEAYAMALS KIYTFGPTFR AENSNTTRHA 250 260 270 280 290 300 SEFWMIEPEM AFYKLNDNIT LAEDLLKYLL SSILNECSQD MDFLENYIEK GLIKKLENVI 310 320 330 340 350 360 NSNFEVITYT KAIEILESSK KNFEIKPYWG IDLQTEHERF LTEETFKKPV VVIDYPKNFK 370 380 390 400 410 420 AFYMKINKDN KTVKGMDVLV PKIGEIIGGS EREDNLQKLE NRIKELNLSI EHLNWYLDLR 430 440 450 460 RFGSTPHSGF GLGLERFVQY STGISNIRDS IPFPRTPKNL YF

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH01365.1.
RefSeq	YP_709541.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227349.
GenomeReviews	Gene locus BAPKO_0102 in contig CP000395_GR.
KEGG	baf:BAPKO_0102.
NMPDR	fig|390236.5.peg.166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SP63.
OMA	Q0SP63. LQKKRHS.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0102-MON.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BORAP

Accession

Primary (citable) accession number: Q0SP63

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)