Reviewed, UniProtKB/Swiss-Prot Q0SP28 (SYH_BORAP)

Names and origin

Protein names

Recommended name:
    Histidyl-tRNA synthetase
    EC=6.1.1.21
Alternative name(s):
    Histidine--tRNA ligase
      Short name=HisRS

Gene names

Name:	hisS
Ordered Locus Names:	BAPKO_0137

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP MF_00127
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP histidine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Histidyl-tRNA synthetase HAMAP MF_00127		PRO_1000016316

Sequences

	Sequence		Length	Mass (Da)
	Q0SP28-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 89BF2C1B86106692	FASTA	456	52,781
	10 20 30 40 50 60 MDIKTLKGFK DYLPKDSLIR IHIVRQIFSV LNSYNFDLID TPVLEYSELL LKKSGDEAEK 70 80 90 100 110 120 QIYRFKDNGG RDVSMRFDLT VPFARFIATN ASNLKFPFRR SQFGKVFRGE NSQKGRYREF 130 140 150 160 170 180 MQFDFDIVGE DSFRGDAEIL SVVYYGLEEI FLNFIEGINK KFIIHYSHLG ILNSFFEKLG 190 200 210 220 230 240 IKEKSLFILR NIDKIDKIGV DKVKEALLLK IEKEVVDSIL NLVNLQGTFK EKIQALKSIL 250 260 270 280 290 300 GDNESVKRVE DVFQHLSLLK IQDSFNLNLK ISRGLDYYTG IVFESEVFDS NMGSVCSGGR 310 320 330 340 350 360 YDNLVSSFSS SIQKISGVGG SFGVDRIKDI IDLEKFSYVK IFVTKARSKV LIVNLDSALQ 370 380 390 400 410 420 DYYYELATRF RNHDYSKVKN ISCEVYFKNK NGKNIKEQIE YALSKEIRFL VFVGQEEYKE 430 440 450 NKMKVRDLTK KEELLLSFEE SINVIKCSEK LLCTPF

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH01400.1.
RefSeq	YP_709576.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227378.
GenomeReviews	Gene locus BAPKO_0137 in contig CP000395_GR.
KEGG	baf:BAPKO_0137.
NMPDR	fig|390236.5.peg.201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SP28.
OMA	Q0SP28. RYDLTIP.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0137-MON.
Family and domain databases
HAMAP	MF_00127. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR015807. His-tRNA-synth_IIa_sub. IPR004516. His-tRNA_synth_IIA. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHER	PTHR11476. His-tRNA_synth. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001549. His-tRNA_synth. 1 hit.
TIGRFAMs	TIGR00442. hisS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYH_BORAP

Accession

Primary (citable) accession number: Q0SP28

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)