Reviewed, UniProtKB/Swiss-Prot Q0SNU2 (SYA_BORAP)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BAPKO_0228

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347510

Sequences

	Sequence		Length	Mass (Da)
	Q0SNU2-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: B421D9E684186517	FASTA	594	67,983
	10 20 30 40 50 60 MTLDKLRKKY IDFFKSKKHF EIMGKSLVPE NDPTVLFNTA GMQPLIPYLL GEVHPSGDML 70 80 90 100 110 120 VNVQKCLRTG DIDEVGDLSH LTFFEMLGNW SLGAYFKEYS VKCSFEFLTS SEYLNIPKDR 130 140 150 160 170 180 LYVSVFEGDQ EIPRDTETAK VWESLGIPKD RIYYLSKDHN FWGPVGSKGP CGPDTEIYVD 190 200 210 220 230 240 TGKIKCSINC NVTCSCGKYF EIWNNVFMQY NKDENGNYME LDRKCVDTGM GLERTIAFLQ 250 260 270 280 290 300 GKSSVYDTDA FMPIIKRIEF ISGKIYGQKE DDDRCIRIIS DHIKAACFIL ADSSGVFPSN 310 320 330 340 350 360 LGQGYVLRRL IRRSIRYAKK LGIKSHFLAD LVDSVETIYR SFYNELTEKK DFIKKELSTE 370 380 390 400 410 420 EEKFFKTLFQ GEQEFIKITR NLSSKTIPGD IAFKLYDTYG FPYELTEELA FEYGFDIDKS 430 440 450 460 470 480 GFNEYFKKHQ KTSKKGGDKV FKGGLADYTY ETTKLHTATH LLHKALQLVL GDHVKQKGSN 490 500 510 520 530 540 ITAERLRFDF VHSKKMTDDE IKKVEDIVNL QIKNSLSVKK SIMELSEARE KGAMALFGEK 550 560 570 580 590 YDNLVSVYEI DGFSLEVCGG PHVENTNELG TFKIQKEQSS SSGIRRIKAI LIDE

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH01486.1.
RefSeq	YP_709662.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227838.
GenomeReviews	Gene locus BAPKO_0228 in contig CP000395_GR.
KEGG	baf:BAPKO_0228.
NMPDR	fig|390236.5.peg.283.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SNU2.
OMA	Q0SNU2. DIDEVGD.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0228-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BORAP

Accession

Primary (citable) accession number: Q0SNU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)