Reviewed, UniProtKB/Swiss-Prot Q0SNR0 (SYL_BORAP)

Names and origin

Protein names

Recommended name:
    Leucyl-tRNA synthetase
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	BAPKO_0261

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	840 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049
Subcellular location	Cytoplasm. HAMAP MF_00049
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 840	840	Leucyl-tRNA synthetase HAMAP MF_00049		PRO_1000009299
Regions
	Motif	44 – 55	12	"HIGH" region HAMAP MF_00049
	Motif	617 – 621	5	"KMSKS" region HAMAP MF_00049
Sites
	Binding site	620	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SNR0-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: B488A7FF899B0BB3	FASTA	840	98,167
	10 20 30 40 50 60 MSKYEFIKIE KKWQEFWDNN KTYKVIEDPN IPKEKRLYIL DMFPYPSANG LHVGHPEGYT 70 80 90 100 110 120 ATDIFARYKI LNGFHVLHPI GFDSFGLPAE NYAIQTGTHP KKSTEENINK FKKQIKALGF 130 140 150 160 170 180 AYDWDREIRT HDENYYKWTQ WIFLQLYKKG LAYAKEMPVW YCPELGTVLA NEEIIQTPNG 190 200 210 220 230 240 PKSERGFHNV EKKYLRQWVL KITKYAERLL NDLEELEWPE SVKEMQRNWI GKSTGVEIDF 250 260 270 280 290 300 EIEGYNDKIK VFTTRPDTIF GITYLVIAPE NKLVEKITKD NFKSNVLKYI KQEELKSDLK 310 320 330 340 350 360 RTSLEKDKSG VFTGSYAFHP ITNVKIPIWV GSYVLGTYGS GAVMGVPAHD ERDFQFAKKY 370 380 390 400 410 420 KLKILPVISK SGKNEILEKA FINDGISINS PDEFNNLKNS EVKDKVIKWL TKNKKGKETV 430 440 450 460 470 480 TYKLRDWVFS RQRYWGEPIP ILFDKLGNAI PLEKNDLPLK LPETANYKPS GTGESPLSRI 490 500 510 520 530 540 KNWVNVKDTD FTRETNTMPQ WAGSCWYYLR YLDPKNSKEF ANHKKIEYWM PVDLYIGGAE 550 560 570 580 590 600 HTVLHLLYSR FWHKVLYDLG HVNTKEPFKK LINQGIITAF SYQKENGVLI PNDQVIEKDN 610 620 630 640 650 660 KFFDKKDNKE VTQVIAKMSK SLKNVINPDG IIKEFGADSI RIYEMFMGPL TDSKPWNTKG 670 680 690 700 710 720 IIGVFRFLNK IWNLREKELS KDNPPKEIMS QLHKVIKKVT EDTEKLNFNT AISAMMIFIN 730 740 750 760 770 780 ELSKYEKNYL NIFKPFIIIL SPYAPHLAEE LWEYIGETPS LFKNSKWPKF DETLIIKETK 790 800 810 820 830 840 EIVLQINGKI KDKILLNKET DEEELKEIAM ENSKIKSNLF NKKIVKIIVI KNKLVNIVIK

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH01518.1.
RefSeq	YP_709694.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227469.
GenomeReviews	Gene locus BAPKO_0261 in contig CP000395_GR.
KEGG	baf:BAPKO_0261.
NMPDR	fig|390236.5.peg.312.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SNR0.
OMA	Q0SNR0. YYLRFID.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0261-MON.
Family and domain databases
HAMAP	MF_00049. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_BORAP

Accession

Primary (citable) accession number: Q0SNR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)