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Q0SNR0 (SYL_BORAP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BAPKO_0261, BafPKo_0253
OrganismBorrelia afzelii (strain PKo) [Complete proteome] [HAMAP]
Taxonomic identifier390236 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group

Protein attributes

Sequence length840 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 840840Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009299

Regions

Motif44 – 5512"HIGH" region HAMAP-Rule MF_00049
Motif617 – 6215"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0SNR0 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: B488A7FF899B0BB3

FASTA84098,167
        10         20         30         40         50         60 
MSKYEFIKIE KKWQEFWDNN KTYKVIEDPN IPKEKRLYIL DMFPYPSANG LHVGHPEGYT 

        70         80         90        100        110        120 
ATDIFARYKI LNGFHVLHPI GFDSFGLPAE NYAIQTGTHP KKSTEENINK FKKQIKALGF 

       130        140        150        160        170        180 
AYDWDREIRT HDENYYKWTQ WIFLQLYKKG LAYAKEMPVW YCPELGTVLA NEEIIQTPNG 

       190        200        210        220        230        240 
PKSERGFHNV EKKYLRQWVL KITKYAERLL NDLEELEWPE SVKEMQRNWI GKSTGVEIDF 

       250        260        270        280        290        300 
EIEGYNDKIK VFTTRPDTIF GITYLVIAPE NKLVEKITKD NFKSNVLKYI KQEELKSDLK 

       310        320        330        340        350        360 
RTSLEKDKSG VFTGSYAFHP ITNVKIPIWV GSYVLGTYGS GAVMGVPAHD ERDFQFAKKY 

       370        380        390        400        410        420 
KLKILPVISK SGKNEILEKA FINDGISINS PDEFNNLKNS EVKDKVIKWL TKNKKGKETV 

       430        440        450        460        470        480 
TYKLRDWVFS RQRYWGEPIP ILFDKLGNAI PLEKNDLPLK LPETANYKPS GTGESPLSRI 

       490        500        510        520        530        540 
KNWVNVKDTD FTRETNTMPQ WAGSCWYYLR YLDPKNSKEF ANHKKIEYWM PVDLYIGGAE 

       550        560        570        580        590        600 
HTVLHLLYSR FWHKVLYDLG HVNTKEPFKK LINQGIITAF SYQKENGVLI PNDQVIEKDN 

       610        620        630        640        650        660 
KFFDKKDNKE VTQVIAKMSK SLKNVINPDG IIKEFGADSI RIYEMFMGPL TDSKPWNTKG 

       670        680        690        700        710        720 
IIGVFRFLNK IWNLREKELS KDNPPKEIMS QLHKVIKKVT EDTEKLNFNT AISAMMIFIN 

       730        740        750        760        770        780 
ELSKYEKNYL NIFKPFIIIL SPYAPHLAEE LWEYIGETPS LFKNSKWPKF DETLIIKETK 

       790        800        810        820        830        840 
EIVLQINGKI KDKILLNKET DEEELKEIAM ENSKIKSNLF NKKIVKIIVI KNKLVNIVIK 

« Hide

References

[1]"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity."
Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M.
BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PKo.
[2]"Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates."
Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E.
J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PKo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000395 Genomic DNA. Translation: ABH01518.1.
CP002933 Genomic DNA. Translation: AEL69479.1.
RefSeqYP_005592469.1. NC_017238.1.
YP_709694.1. NC_008277.1.

3D structure databases

ProteinModelPortalQ0SNR0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING390236.BAPKO_0261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABH01518; ABH01518; BAPKO_0261.
AEL69479; AEL69479; BafPKo_0253.
GeneID12159207.
4227469.
KEGGbaf:BAPKO_0261.
bafz:BafPKo_0253.
PATRIC37188810. VBIBorAfz3878_0317.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BORAP
AccessionPrimary (citable) accession number: Q0SNR0
Secondary accession number(s): G0IR93
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries