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Q0SNE1

- SYE_BORAP

UniProt

Q0SNE1 - SYE_BORAP

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Borrelia afzelii (strain PKo)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei254 – 2541ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:BAPKO_0381, BafPKo_0371
    OrganismiBorrelia afzelii (strain PKo)
    Taxonomic identifieri390236 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000001825: Chromosome, UP000005216: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Glutamate--tRNA ligasePRO_1000001875Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi390236.BAPKO_0381.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0SNE1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi9 – 1911"HIGH" regionAdd
    BLAST
    Motifi251 – 2555"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiVTGQTHG.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0SNE1-1 [UniParc]FASTAAdd to Basket

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    MGIRVRYAPS PTGLQHIGGI RTALFNYFFA KSCGGKFLLR IEDTDQSRYF    50
    SEAENDLYSS LKWLGISFDE GPVVGGDYAP YVQSQRSAIY KRYAEYLIES 100
    GHAYYCYCSP ERLERIKKIQ NINKMPPGYD RHCRNLSDEE IENVLIKKIK 150
    PVVRFKIPLE GDTSFDDVLL GKITWSNKDI SPDPVILKSD GLPTYHLANV 200
    VDDYLMKITH VLRAQEWVSS GPLHTLLYKA FKWNPPIYCH LPMVMGNDGQ 250
    KLSKRHGSTA LRQFIEDGYL PEAIINYITL LGWSYDDKRE FFSKSDLEKF 300
    FSIEKINKSP AIFDYHKLDF FNSYYIREKK DEDLFNLLLP FFQKKGYVSK 350
    PNTLEESQKL KLLVPLIKSR IKKLSDALSM TKFFYEDIKS WNLDEFLGRK 400
    KTAKEVCSIL ELIKPILEGF EKRSLEENDK IFYDFAKNNG FKLGEILLPI 450
    RIAVLGSKVS PPLFDSLKLI GKSKVFERNK LAQEFLRINE 490
    Length:490
    Mass (Da):56,815
    Last modified:September 5, 2006 - v1
    Checksum:iA4441D298C9F7779
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti479 – 4791N → I in AEL69597. (PubMed:22123755)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000395 Genomic DNA. Translation: ABH01637.1.
    CP002933 Genomic DNA. Translation: AEL69597.1.
    RefSeqiWP_011600986.1. NC_008277.1.
    YP_005592587.1. NC_017238.1.
    YP_709813.1. NC_008277.1.

    Genome annotation databases

    EnsemblBacteriaiABH01637; ABH01637; BAPKO_0381.
    AEL69597; AEL69597; BafPKo_0371.
    GeneIDi12159325.
    4227560.
    KEGGibaf:BAPKO_0381.
    bafz:BafPKo_0371.
    PATRICi37189046. VBIBorAfz3878_0435.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000395 Genomic DNA. Translation: ABH01637.1 .
    CP002933 Genomic DNA. Translation: AEL69597.1 .
    RefSeqi WP_011600986.1. NC_008277.1.
    YP_005592587.1. NC_017238.1.
    YP_709813.1. NC_008277.1.

    3D structure databases

    ProteinModelPortali Q0SNE1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 390236.BAPKO_0381.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABH01637 ; ABH01637 ; BAPKO_0381 .
    AEL69597 ; AEL69597 ; BafPKo_0371 .
    GeneIDi 12159325.
    4227560.
    KEGGi baf:BAPKO_0381.
    bafz:BafPKo_0371.
    PATRICi 37189046. VBIBorAfz3878_0435.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi VTGQTHG.
    OrthoDBi EOG6DRPF7.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity."
      Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M.
      BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PKo.
    2. "Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates."
      Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E.
      J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PKo.

    Entry informationi

    Entry nameiSYE_BORAP
    AccessioniPrimary (citable) accession number: Q0SNE1
    Secondary accession number(s): G0IS16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3