Q0SNA5 (SYP_BORAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	BAPKO_0419

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Proline--tRNA ligase HAMAP MF_01571		PRO_0000288404

Sequences

	Sequence		Length	Mass (Da)
	Q0SNA5 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: EDD310D875662AE0	FASTA	488	56,265
	10 20 30 40 50 60 MSDFIASKED DYSKWYLDIV QKAKLADYSP VKGCMVIMPY GYSIWSKIQS ILDKKFKETG 70 80 90 100 110 120 HENAYFPMLI PYGFLEKEKD HIDGFSPEFA IIKDAGGESL VEPLVLRPTS ETIIWNMYSK 130 140 150 160 170 180 WIKSYRDLPL KINQWANVIR WEKRTRPFLR TTEFLWQEGH TAHATEEEAL EETLLILDVY 190 200 210 220 230 240 KRFMEDYLAI PVFCGKKSEN EKFAGAVSTY SVEALMQDKK ALQAATSHYL GLNFAKAFDV 250 260 270 280 290 300 KFQDKDGKMK HVFASSWGVS TRLIGALIMV HSDEKGLILP PRIAPVEIIV IPIFKKEDEI 310 320 330 340 350 360 NKKILDYSDC VVHTLKKAEF RVEIDKDVRS SPGFRFSSAE FKGIPIRIEV GINDILLNSV 370 380 390 400 410 420 TIIRRDKDRK FKYQISLDSL VSKVRVELDS MQKDLFKKAL NFRTLNTKEI FRSGKDSYEL 430 440 450 460 470 480 FKAYVNDYSG FVLSCWCGGL NCENIIKNET KATIRCIPDD FKARDLTGMT CIYCSSKAKY YVLFAKSY

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000395 Genomic DNA. Translation: ABH01673.1.
RefSeq	YP_709849.1. NC_008277.1.
3D structure databases
ProteinModelPortal	Q0SNA5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SNA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000004287; EBBORP00000003905; EBBORG00000004287.
GeneID	4227615.
GenomeReviews	Gene locus BAPKO_0419 in contig CP000395_GR.
KEGG	baf:BAPKO_0419.
NMPDR	fig|390236.5.peg.466.
PATRIC	37189122. VBIBorAfz3878_0471.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
GeneTree	EBGT00050000007260.
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0419-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BORAP

Accession

Primary (citable) accession number: Q0SNA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	September 19, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program