Q0SN68 (RNPA_BORAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein component
Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5

Alternative name(s):
Protein C5

Gene names

Name:	rnpA
Ordered Locus Names:	BAPKO_0463, BafPKo_0452

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group › Borrelia afzelii

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme By similarity. HAMAP-Rule MF_00227
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or RnpB) and a protein subunit By similarity.
Sequence similarities	Belongs to the RnpA family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 111	111	Ribonuclease P protein component HAMAP-Rule MF_00227		PRO_1000021380

Sequences

	Sequence		Length	Mass (Da)
	Q0SN68 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 543DE32C7001DCC6	FASTA	111	13,343
	10 20 30 40 50 60 MKKRNISLKS KIEIQKIFKE GNLIRFSNLN LKMFCKSNHL IYSRLLVTFS KSFRGSVKRN 70 80 90 100 110 RVRRLFKEAF RKRLELLEGR AIDIIFVVYY DRLDLTYFSI ESLMKSLVLM V

References

[1]	"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.
[2]	"Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates." Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E. J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000395 Genomic DNA. Translation: ABH01710.1. CP002933 Genomic DNA. Translation: AEL69664.1.
RefSeq	YP_005592654.1. NC_017238.1. YP_709886.1. NC_008277.1.
3D structure databases
ProteinModelPortal	Q0SN68.
ModBase	Search...
Protein-protein interaction databases
STRING	390236.BAPKO_0463.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABH01710; ABH01710; BAPKO_0463. AEL69664; AEL69664; BafPKo_0452.
GeneID	12159401. 4227590.
KEGG	baf:BAPKO_0463. bafz:BafPKo_0452.
PATRIC	37189200. VBIBorAfz3878_0504.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0594.
HOGENOM	HOG000221236.
KO	K03536.
OMA	RIFKECF.
ProtClustDB	CLSK507595.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00227. RNase_P.
InterPro	IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR000100. RNase_P. [Graphical view]
Pfam	PF00825. Ribonuclease_P. 1 hit. [Graphical view]
ProDom	PD003629. Ribonuclease_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00188. rnpA. 1 hit.
PROSITE	PS00648. RIBONUCLEASE_P. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPA_BORAP

Accession

Primary (citable) accession number: Q0SN68
Secondary accession number(s): G0IS83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program