Q0SN63 (SYD_BORAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BAPKO_0468

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000006640

Sequences

	Sequence		Length	Mass (Da)
	Q0SN63 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: FBE8B893802988DC	FASTA	586	68,229
	10 20 30 40 50 60 MFKVIKCNEL NEKLINKKVE INAWVKKIRH HGKFTFLDIR DRYEKAQVLI TEEHLLKIVE 70 80 90 100 110 120 KIKLEYCIKI QGLLIKRPSN MINANMTTGY FEILAKNIEI ISKCNELPFM IEDDNNASEN 130 140 150 160 170 180 SKLKYRYLDL RRDSLKNKII LRCQATHLIR NFLVKRKFLE LETPTFVKST PEGARDFVIP 190 200 210 220 230 240 SRIHKGSFYA LPQSPQLYKQ LIMIAGFDKY FQIARCYRDE DSRGDRQPEF TQLDLEMSFV 250 260 270 280 290 300 KKENIFKLIE NMLFSIFKNC LNIKLSKKFK KITYKTAMNK YGSDKPDTRF ELTLQDISRN 310 320 330 340 350 360 LKNSEFNVFK DILNNKGSIK TLIVKDKADT FSRAKINNLE EIAKLYKTQG LYFTKIENNK 370 380 390 400 410 420 FSGGIAKFLK TEEQQLIKAY SLENNDIIFF IANNKWEIAC KAMGQIRIKI ANDLGLIDEN 430 440 450 460 470 480 KFEFLWVYDF PLFEYDENTK TYIPAHHMFS IPKKRYISNL EKNPNKAIGE IYDLVLNGVE 490 500 510 520 530 540 LGSGSIRIHN KELQQRIFNI IGFQKEKSED RFGFFLKALE YGAPNHGGIA IGIDRLIMLM 550 560 570 580 TKSTSIKDVI LFPKNSFAAS PLDNSPSKIS NEQLKELGIN IAPDDT

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000395 Genomic DNA. Translation: ABH01715.1.
RefSeq	YP_709891.1. NC_008277.1.
3D structure databases
ProteinModelPortal	Q0SN63.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SN63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000004326; EBBORP00000003944; EBBORG00000004326.
GeneID	4227768.
GenomeReviews	Gene locus BAPKO_0468 in contig CP000395_GR.
KEGG	baf:BAPKO_0468.
NMPDR	fig|390236.5.peg.508.
PATRIC	37189212. VBIBorAfz3878_0510.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
GeneTree	EBGT00050000007238.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0468-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BORAP

Accession

Primary (citable) accession number: Q0SN63

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program