Q0SN37 (LSPA_BORAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BAPKO_0498, BafPKo_0487

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group › Borrelia afzelii

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP-Rule MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP-Rule MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP-Rule MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Lipoprotein signal peptidase HAMAP-Rule MF_00161		PRO_0000289355
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	72 – 92	21	Helical; Potential
	Transmembrane	96 – 118	23	Helical; Potential
	Transmembrane	143 – 163	21	Helical; Potential
Sites
	Active site	114	1	By similarity
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SN37 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: F3FA45255BA78897	FASTA	170	19,980
	10 20 30 40 50 60 MSVKSKQYFN IFIFIISLIF FDQLSKYLVV KYVKLGSVYF SFFENFFRII HVRNTGILFS 70 80 90 100 110 120 IGSNINYSLK KIFFLAMPIF ILIFIFSLSL KEKNRIARIS LLLIFSGGVG NVIDRLFRPS 130 140 150 160 170 GVVDFLDFKF YGIFGLDRWP TFNFADSYVV IGMILFLVYD FFIKRKAFNT

References

[1]	"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.
[2]	"Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates." Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E. J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000395 Genomic DNA. Translation: ABH01741.1. CP002933 Genomic DNA. Translation: AEL69695.1.
RefSeq	YP_005592685.1. NC_017238.1. YP_709917.1. NC_008277.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	390236.BAPKO_0498.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABH01741; ABH01741; BAPKO_0498. AEL69695; AEL69695; BafPKo_0487.
GeneID	12159435. 4227949.
KEGG	baf:BAPKO_0498. bafz:BafPKo_0487.
PATRIC	37189271. VBIBorAfz3878_0535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0597.
HOGENOM	HOG000096992.
KO	K03101.
OMA	KYLVVKY.
ProtClustDB	PRK01574.
Enzyme and pathway databases
UniPathway	UPA00665.
Family and domain databases
HAMAP	MF_00161. LspA.
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BORAP

Accession

Primary (citable) accession number: Q0SN37
Secondary accession number(s): G0ISB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program