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Q0SMV7 (DNLJ_BORAP) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Ordered Locus Names:BAPKO_0581
OrganismBorrelia afzelii (strain PKo) [Complete proteome] [HAMAP]
Taxonomic identifier390236 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length660 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 660660DNA ligase HAMAP MF_01588
PRO_0000313147

Regions

Domain583 – 66078BRCT
Nucleotide binding33 – 375NAD By similarity
Nucleotide binding82 – 832NAD By similarity

Sites

Active site1121N6-AMP-lysine intermediate By similarity
Metal binding3961Zinc By similarity
Metal binding3991Zinc By similarity
Metal binding4121Zinc By similarity
Metal binding4171Zinc By similarity
Binding site1101NAD By similarity
Binding site1331NAD By similarity
Binding site1671NAD By similarity
Binding site2811NAD By similarity
Binding site3051NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0SMV7 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 6784794051042F07

FASTA66075,435
        10         20         30         40         50         60 
MSSKIQQEIA DLKKLIRKWD KEYYVDSLPS VEDFVYDKHI LRLQELESKC PEYKTLDSPT 

        70         80         90        100        110        120 
LKFGSDLLND FKEVEHSVPV LSLDKVYNLD LLKSWIDKID FSNSFNISVE PKIDGCSIVL 

       130        140        150        160        170        180 
YYKDGILEKA LTRGNGKFGN DVTKNIRTIR HVPLFLGEKV DLVLRGEVYI TKENFLKINK 

       190        200        210        220        230        240 
FLEKPYTNSR NLASGILRRV DSREVANFPL NIFIYDFLNA GLDFKTNNLA IEKLKKLGFK 

       250        260        270        280        290        300 
VNPLIRFFDQ KSSIKEVLNY IADITKKRNS FEYEIDGVVL KVSDFALREK LGYTLHHPKW 

       310        320        330        340        350        360 
AMAYKFEALS GFSKVNSIVV QVGRSGKITP VANIDKVFVS GAFITNATLH NQDYITSINL 

       370        380        390        400        410        420 
NVGDIVKVSR RGDVIPAVEM VINKFSTGFF KVPDKCPSCK TVVVKEGAHF FCTNNNCPSV 

       430        440        450        460        470        480 
AVERIKYFCS KNCMDIEGFS DKTISFLFEK KFISSEIDLY TFNFYKLLKF KGFKDRKINN 

       490        500        510        520        530        540 
LINSIEASKK KPFSKLLLSI GIKELGENTI RLLFLNNLNS FSKLFRLCQD RDFAFLTLLK 

       550        560        570        580        590        600 
IKGIGEKIAL NIIDAFNNSI MINKFKVFEN LGFKMEERIL IDDENKLLVG KKFCITGSFN 

       610        620        630        640        650        660 
GYSRPIVIDK LENKGAIFKT CVTKGLDFLV VGEKAGTKLK KALNLNVKIM SFEDIKSYLD

« Hide

References

[1]"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity."
Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M.
BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PKo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000395 Genomic DNA. Translation: ABH01821.1.
RefSeqYP_709997.1. NC_008277.1.

3D structure databases

ProteinModelPortalQ0SMV7.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0SMV7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBORT00000004109; EBBORP00000003727; EBBORG00000004109.
GeneID4227116.
GenomeReviewsGene locus BAPKO_0581 in contig CP000395_GR.
KEGGbaf:BAPKO_0581.
NMPDRfig|390236.5.peg.607.
PATRIC37189431. VBIBorAfz3878_0612.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0272.
GeneTreeEBGT00050000007352.
HOGENOMHBG620317.
OMAPEYICEL.
ProtClustDBPRK14350.

Enzyme and pathway databases

BioCycBAFZ390236:BAPKO_0581-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00278. HhH1. 3 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. False negative.
PS01056. DNA_LIGASE_N2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_BORAP
AccessionPrimary (citable) accession number: Q0SMV7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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