Reviewed, UniProtKB/Swiss-Prot Q0SMT3 (PYRG_BORAP)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	BAPKO_0606

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	CTP synthase HAMAP MF_01227		PRO_0000266076
Regions
	Domain	304 – 533	230	Glutamine amidotransferase type-1
	Region	1 – 256	256	Aminator domain HAMAP MF_01227
Sites
	Active site	382	1	Nucleophile By similarity
	Active site	511	1	By similarity
	Active site	513	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SMT3-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 84E463023F3269A1	FASTA	533	59,586
	10 20 30 40 50 60 MKKNLKILVI TGGVISGIGK GVTSASIARL FRYDFRVTPI KCDGYLNTDP GTINPVEHGE 70 80 90 100 110 120 VFVLDDGGEV DMDFGHYERF LNLNAKSSWN ITMGKIYKKI LENERKGKYL GRTVQLIPHV 130 140 150 160 170 180 TDEIKSTIFQ IASSENSEML IIEIGGTVGD MENILFIETV RQIRHEVGSG NIAFIHLTYV 190 200 210 220 230 240 PSPVGINEQK SKPTQQSVKT LNKAGIFPDL IIARSSQVLT DQIRKKIAMF CNVESTSIID 250 260 270 280 290 300 NIDVSTIYEI PISFYKQGVH EILSSKLNIK VDPKIEELSR LVGIIKSNFF VPKKIINIAI 310 320 330 340 350 360 CGKYAELDDS YASIRESLVH VAANLDLLIK STLIDSNDLN ESCLKDFDGI IVPGGFGGKG 370 380 390 400 410 420 YEGKIIAIKY ARENNIPFLG ICLGLQLAVI EFARNVCGIL DADTEENLIK DKPLKNPVIH 430 440 450 460 470 480 LLPEQKEIKD KGATMRLGGY PVILKKNTIA FKLYGQDRII ERFRHRYEVN NDYIDLFEKN 490 500 510 520 530 GLIVSGFSSD FKIAKLIEIP KNKFFVACQF HPELITRIEN PAKLFLGLIK ACI

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH01845.1.
RefSeq	YP_710021.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227309.
GenomeReviews	Gene locus BAPKO_0606 in contig CP000395_GR.
KEGG	baf:BAPKO_0606.
NMPDR	fig|390236.5.peg.631.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SMT3.
OMA	Q0SMT3. EFNNAYR.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0606-MON.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_BORAP

Accession

Primary (citable) accession number: Q0SMT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)