Reviewed, UniProtKB/Swiss-Prot Q0SMS3 (MURD_BORAP)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BAPKO_0616

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000301416
Regions
	Nucleotide binding	118 – 124	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0SMS3-1 [UniParc]. Last modified September 19, 2006. Version 1. Checksum: 267FCDFA92509B50	FASTA	451	51,147
	10 20 30 40 50 60 MRLDEIKNLN FLVMGLGLNG GGVALSRFLL KHGAKLVITD LKSEAELALS IDSLRDFDDQ 70 80 90 100 110 120 IRYVLGKHDV NDFKKADIVV KNPSVRPNNK YLKLAKRVET DISLFLIFNK NPIIAVTGTK 130 140 150 160 170 180 GKSTLVSLLY QALKKKYPRV KLGGNIGVSP LSFFDQLDGK SPLILELSSW QLQSLENFNP 190 200 210 220 230 240 ILSIITNVYN DHQNYYSNFD DYIIDKSKIF VNQTSGIVII QDKAYYKYFS KFESKAKVIL 250 260 270 280 290 300 FSEFNPCNLD QDIFYSNKGE VYFNDNLIGS FFESQVVFMI PKLIAFFVAY YLNIDLNHMF 310 320 330 340 350 360 QILKNFKGIE HRLEFVKLVR NVMFYNDTAS TIPDSTVLSV KSLKTNDNCI NLIVGGTDKN 370 380 390 400 410 420 LDFSSFSKII NLVKAWILIK GSATVKIINV LEKSSIQYFV FDSLRGAVNY AFEISSPGDI 430 440 450 VLFSPASASF ELFNNEFDRG LQFKKLVDML G

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH01855.1.
RefSeq	YP_710031.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227919.
GenomeReviews	Gene locus BAPKO_0616 in contig CP000395_GR.
KEGG	baf:BAPKO_0616.
NMPDR	fig|390236.5.peg.641.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SMS3.
OMA	Q0SMS3. VSPLSFF.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0616-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BORAP

Accession

Primary (citable) accession number: Q0SMS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 19, 2006
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)