Reviewed, UniProtKB/Swiss-Prot Q0SMS1 (SYM_BORAP)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BAPKO_0618

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331786
Regions
	Domain	560 – 665	106	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	330 – 334	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	155	1	Zinc By similarity
	Metal binding	158	1	Zinc By similarity
	Binding site	333	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SMS1-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 3D8839B7529646D9	FASTA	724	84,391
	10 20 30 40 50 60 MKKMNLITAA LPYVNNIPHL GNLVQVLSAD AFARYSKMSG IETLYICGTD EYGTATETKA 70 80 90 100 110 120 LIEKTTPLEL CNKYYEIHKS IYKWFNIEFD IFGRTTNKHH QETVQNFFLK LEKNGYIKER 130 140 150 160 170 180 ETEQFFCNKD LIFLADRYVI GECPECQSMA KGDQCDNCSK LLNPTDLINP KCIICKNKPI 190 200 210 220 230 240 LKKTNHLYID LPKIKTKLEK WIKNPTTSKN WNTNALKMTN AFLRDGLKER AITRDLKWGI 250 260 270 280 290 300 PVPKKGYENK VFYVWFDAPI GYISITKNIV KNWESWWKNN KQVNLVQFIG KDNILFHTIM 310 320 330 340 350 360 FPCIKIGSKE NWTTLNQLSS SEYLNYENLK FSKSEGTGIF GNDVITTGIP SDVWRFYIYY 370 380 390 400 410 420 NRPEKSDFQF IWQDLMERVN TELIDNFSNL VNRVLTFQKK FFGDVIETIE IQHKFWEQIT 430 440 450 460 470 480 PKYNKILNLF KNTELKSALK EILKISSFGN KIFQDNEPWK RKESSPQEIK ELILNLIYLI 490 500 510 520 530 540 RDLSILMMPF IPETSKKIQQ FFGNSYQFST KILGTKSGIK KIEFVEILFN KLEQKKINDL 550 560 570 580 590 600 RLKYSGEKNM KEKEQPENLF REKVLLRVVK INKIERNPEA KNLFILKLDD GTNKDKQIVS 610 620 630 640 650 660 SLEGYYTEEE LLGKHIIIVD NLKPAKFRGI KSEGMLIAAE DKNKNFKIII VEDSIKNPIA 670 680 690 700 710 720 GERIILENDQ NKDLTCPPKI DINKFSKANI ITENGELKIN GINLILEHSK NKILSKDIPN GIVC

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH01857.1.
RefSeq	YP_710033.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227921.
GenomeReviews	Gene locus BAPKO_0618 in contig CP000395_GR.
KEGG	baf:BAPKO_0618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SMS1.
OMA	Q0SMS1. SAYQPEQ.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0618-MON.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BORAP

Accession

Primary (citable) accession number: Q0SMS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)