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Protein

Ribonuclease 3

Gene

rnc

Organism
Borrelia afzelii (strain PKo)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591MagnesiumUniRule annotation
Active sitei63 – 631UniRule annotation
Metal bindingi132 – 1321MagnesiumUniRule annotation
Active sitei135 – 1351UniRule annotation
Metal bindingi135 – 1351MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:BafPKo_0728, BAPKO_0748
OrganismiBorrelia afzelii (strain PKo)
Taxonomic identifieri390236 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBorreliaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000005216 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Ribonuclease 3PRO_1000075728Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi390236.BAPKO_0748.

Structurei

3D structure databases

ProteinModelPortaliQ0SMF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 146129RNase IIIUniRule annotationAdd
BLAST
Domaini173 – 24270DRBMUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
Contains 1 RNase III domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246808.
KOiK03685.
OMAiSADHNER.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0SMF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNSSDFCF SNERKAQLSE FLENLSIDFN NFDLLNTALS HSSYSNELDQ
60 70 80 90 100
KSDNNERLEF LGDSVLNLII TDHLYKTYPN KSEGELSKAR SYIVSEDSLS
110 120 130 140 150
SIAREIDLGS YILLGRGEES NDGRNKKGIL ADAIEAFVGA IYLDSGFLIA
160 170 180 190 200
TEFVVGLFDM YIRLMFNRGD FKDYKSLLQE YVQKKYKISP NYKLDKEIGP
210 220 230 240
DHDKVFCVEL YVGEKFISNG KGKSKKEAEM RAAEVALKAM EDINL
Length:245
Mass (Da):27,837
Last modified:September 5, 2006 - v1
Checksum:i93B257858F92DC85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000395 Genomic DNA. Translation: ABH01978.1.
CP002933 Genomic DNA. Translation: AEL69923.1.
RefSeqiWP_011601177.1. NC_017238.1.
YP_005592913.1. NC_017238.1.
YP_710154.1. NC_008277.1.

Genome annotation databases

EnsemblBacteriaiABH01978; ABH01978; BAPKO_0748.
AEL69923; AEL69923; BafPKo_0728.
KEGGibaf:BAPKO_0748.
bafz:BafPKo_0728.
PATRICi37189753. VBIBorAfz3878_0763.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000395 Genomic DNA. Translation: ABH01978.1.
CP002933 Genomic DNA. Translation: AEL69923.1.
RefSeqiWP_011601177.1. NC_017238.1.
YP_005592913.1. NC_017238.1.
YP_710154.1. NC_008277.1.

3D structure databases

ProteinModelPortaliQ0SMF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390236.BAPKO_0748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABH01978; ABH01978; BAPKO_0748.
AEL69923; AEL69923; BafPKo_0728.
KEGGibaf:BAPKO_0748.
bafz:BafPKo_0728.
PATRICi37189753. VBIBorAfz3878_0763.

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246808.
KOiK03685.
OMAiSADHNER.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity."
    Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M.
    BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PKo.
  2. "Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates."
    Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E.
    J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PKo.

Entry informationi

Entry nameiRNC_BORAP
AccessioniPrimary (citable) accession number: Q0SMF0
Secondary accession number(s): G0IRF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 5, 2006
Last modified: June 24, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.