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Q0SM46

- PNP_BORAP

UniProt

Q0SM46 - PNP_BORAP

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Borrelia afzelii (strain PKo)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi486 – 4861MagnesiumUniRule annotation
    Metal bindingi492 – 4921MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:BAPKO_0858, BafPKo_0833
    OrganismiBorrelia afzelii (strain PKo)
    Taxonomic identifieri390236 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000001825: Chromosome, UP000005216: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 722722Polyribonucleotide nucleotidyltransferasePRO_0000329539Add
    BLAST

    Proteomic databases

    PRIDEiQ0SM46.

    Interactioni

    Protein-protein interaction databases

    STRINGi390236.BAPKO_0858.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0SM46.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini553 – 61260KHUniRule annotationAdd
    BLAST
    Domaini622 – 71796S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OMAiPRWDWVA.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 2 hits.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0SM46-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKILKLKIG RDELVFETGF MAKQANGSVL ATYGGSSVLA TVCCSNNVRE    50
    DLDFVPLSVE YNEKYYAAGK IPGGFIKREG KPKDKEILVS RLIDRPMRPL 100
    FDKRFGREIQ VIPTTLATDQ LNPPDIVGMN AAFTAVFLSD IPFNGPIAAV 150
    RMVYLNGKFI VNPSFEEIHD SDLDIVVAGS LNGITMVEGG ANEVSEDILL 200
    SAIDSAHEYI KQICNAQKEF LDIVGEKEKL PLAFEEKIFE FKEELRDFIY 250
    ADLKEACFVK GKLNRDKAIT LLRNKSYEHF SSLEKLDDSN ESLFYKAFDD 300
    FEKEIVRNSI LNDKIRTDGR TPNEIRDILA EVDILSRTHG SSLFTRGETQ 350
    ALAVTTLGTS IDEQIMDDID GDKRLNFMLH YNFPPFSVGE TGRLMTGRRE 400
    IGHGHLAQRA LESMIPGKND FPYTIRVVSE ILESNGSSSM ATVCAGSMSL 450
    MSAGVPVKRQ VAGIAMGLIS EGDKYVVLSD ILGEEDHLGD MDFKVAGTEN 500
    GITGFQMDIK IENVTKQLMR DALEQARVGR IHILSIMNSV ISNSRVGISK 550
    YAPKIIQLQI DIDKISLVIG STGKTVKAIT DEFEVKVQIE QNGKIILFGD 600
    DDFKMQKAKE KIESIVREPK VGEIYEGTVK KINSFGAFIE LTPTKEGFLS 650
    TRLKSRDSKY GSGRFGHGNR YSRFGSGGDN IRGNAGLVRP PKLEEGQRIK 700
    VRIVDIDKFG KIDLEIVRDK DY 722
    Length:722
    Mass (Da):80,376
    Last modified:September 5, 2006 - v1
    Checksum:i9BCAB9C88DC5E0FB
    GO

    Sequence cautioni

    The sequence AEL70022.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000395 Genomic DNA. Translation: ABH02082.1.
    CP002933 Genomic DNA. Translation: AEL70022.1. Different initiation.
    RefSeqiYP_005593012.1. NC_017238.1.
    YP_710258.1. NC_008277.1.

    Genome annotation databases

    EnsemblBacteriaiABH02082; ABH02082; BAPKO_0858.
    AEL70022; AEL70022; BafPKo_0833.
    GeneIDi12158927.
    4227019.
    KEGGibaf:BAPKO_0858.
    bafz:BafPKo_0833.
    PATRICi37189963. VBIBorAfz3878_0862.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000395 Genomic DNA. Translation: ABH02082.1 .
    CP002933 Genomic DNA. Translation: AEL70022.1 . Different initiation.
    RefSeqi YP_005593012.1. NC_017238.1.
    YP_710258.1. NC_008277.1.

    3D structure databases

    ProteinModelPortali Q0SM46.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 390236.BAPKO_0858.

    Proteomic databases

    PRIDEi Q0SM46.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABH02082 ; ABH02082 ; BAPKO_0858 .
    AEL70022 ; AEL70022 ; BafPKo_0833 .
    GeneIDi 12158927.
    4227019.
    KEGGi baf:BAPKO_0858.
    bafz:BafPKo_0833.
    PATRICi 37189963. VBIBorAfz3878_0862.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OMAi PRWDWVA.
    OrthoDBi EOG6WT8CC.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 2 hits.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity."
      Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M.
      BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PKo.
    2. "Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates."
      Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E.
      J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PKo.

    Entry informationi

    Entry nameiPNP_BORAP
    AccessioniPrimary (citable) accession number: Q0SM46
    Secondary accession number(s): G0IRY7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3