Reviewed, UniProtKB/Swiss-Prot Q0SM46 (PNP_BORAP)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	BAPKO_0858

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 722	722	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329539
Regions
	Domain	553 – 612	60	KH
	Domain	622 – 717	96	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q0SM46-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 9BCAB9C88DC5E0FB	FASTA	722	80,376
	10 20 30 40 50 60 MRKILKLKIG RDELVFETGF MAKQANGSVL ATYGGSSVLA TVCCSNNVRE DLDFVPLSVE 70 80 90 100 110 120 YNEKYYAAGK IPGGFIKREG KPKDKEILVS RLIDRPMRPL FDKRFGREIQ VIPTTLATDQ 130 140 150 160 170 180 LNPPDIVGMN AAFTAVFLSD IPFNGPIAAV RMVYLNGKFI VNPSFEEIHD SDLDIVVAGS 190 200 210 220 230 240 LNGITMVEGG ANEVSEDILL SAIDSAHEYI KQICNAQKEF LDIVGEKEKL PLAFEEKIFE 250 260 270 280 290 300 FKEELRDFIY ADLKEACFVK GKLNRDKAIT LLRNKSYEHF SSLEKLDDSN ESLFYKAFDD 310 320 330 340 350 360 FEKEIVRNSI LNDKIRTDGR TPNEIRDILA EVDILSRTHG SSLFTRGETQ ALAVTTLGTS 370 380 390 400 410 420 IDEQIMDDID GDKRLNFMLH YNFPPFSVGE TGRLMTGRRE IGHGHLAQRA LESMIPGKND 430 440 450 460 470 480 FPYTIRVVSE ILESNGSSSM ATVCAGSMSL MSAGVPVKRQ VAGIAMGLIS EGDKYVVLSD 490 500 510 520 530 540 ILGEEDHLGD MDFKVAGTEN GITGFQMDIK IENVTKQLMR DALEQARVGR IHILSIMNSV 550 560 570 580 590 600 ISNSRVGISK YAPKIIQLQI DIDKISLVIG STGKTVKAIT DEFEVKVQIE QNGKIILFGD 610 620 630 640 650 660 DDFKMQKAKE KIESIVREPK VGEIYEGTVK KINSFGAFIE LTPTKEGFLS TRLKSRDSKY 670 680 690 700 710 720 GSGRFGHGNR YSRFGSGGDN IRGNAGLVRP PKLEEGQRIK VRIVDIDKFG KIDLEIVRDK DY

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH02082.1.
RefSeq	YP_710258.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227019.
GenomeReviews	Gene locus BAPKO_0858 in contig CP000395_GR.
KEGG	baf:BAPKO_0858.
NMPDR	fig|390236.5.peg.860.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SM46.
OMA	Q0SM46. GHGNLAK.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0858-MON.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_BORAP

Accession

Primary (citable) accession number: Q0SM46

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)