Reviewed, UniProtKB/Swiss-Prot Q0SM34 (MURC_BORAP)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BAPKO_0870

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000336817
Regions
	Nucleotide binding	121 – 127	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0SM34-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 04DCD1F0449B9669	FASTA	468	53,553
	10 20 30 40 50 60 MKVDFDSLNN IFFVGIKGSG VCSLACFLNS KGYFVEGVDV SDKFHTDKIL SNNKISYYEN 70 80 90 100 110 120 IYEFSLKELG RSFDLIVYSS AYDKYGLPAL LEAKELNIPI LSYSEVLGEL SRKYYSVGIA 130 140 150 160 170 180 GSHGKTTTTA FLGLLFNKLG LNPNVIVGSS VKDFGDNSAI AGISNIFIAE TCEYKKHFLH 190 200 210 220 230 240 FSPNMLILTN IDYEHVDFFE NYEALEDAFL QYINNLKKNG ILIINSDDNN LLKIKRQINR 250 260 270 280 290 300 KDINIFSFGS KDLSNFQISN IVVKNEYFCF SFLGLCNVEL RTVLFHNVLN FSAALLALNL 310 320 330 340 350 360 FLESNGKSIF DFEEAVKKIA KNYSGIKRRV EVVKEKKGVI YMDDYAHHPR EIRDTLLGIK 370 380 390 400 410 420 DFYKNKRIIL DFMPHTFTRT KEFFNDFVEV LSVADILILH NIYLSNRENF NPDELSVKLF 430 440 450 460 LNIKKINKNT YFFKDVKDSV EFIKSLLISG DLFITMGAGN NFILHDFL

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000395 Genomic DNA. Translation: ABH02094.1.
RefSeq	YP_710270.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4227147.
GenomeReviews	Gene locus BAPKO_0870 in contig CP000395_GR.
KEGG	baf:BAPKO_0870.
NMPDR	fig|390236.5.peg.872.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0SM34.
OMA	Q0SM34. EWMVVEA.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0870-MON.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BORAP

Accession

Primary (citable) accession number: Q0SM34

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)