Q0SM30 (MIAA_BORAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA dimethylallyltransferase
EC=2.5.1.75

Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name=DMAPP:tRNA dimethylallyltransferase
Short name=DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name=IPP transferase
Short name=IPPT
Short name=IPTase

Gene names

Name:	miaA
Ordered Locus Names:	BAPKO_0874

Organism

Borrelia afzelii (strain PKo) [Complete proteome] [HAMAP]

Taxonomic identifier

390236 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Borrelia › Borrelia burgdorferi group

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity. HAMAP MF_00185
Catalytic activity	Dimethylallyl diphosphate + tRNA = diphosphate + tRNA containing 6-dimethylallyladenosine. HAMAP MF_00185
Cofactor	Magnesium By similarity. HAMAP MF_00185
Subunit structure	Monomer By similarity. HAMAP MF_00185
Sequence similarities	Belongs to the IPP transferase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA dimethylallyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	tRNA dimethylallyltransferase HAMAP MF_00185		PRO_1000020566
Regions
	Nucleotide binding	11 – 18	8	ATP Potential
	Region	13 – 18	6	Substrate binding By similarity
	Region	35 – 38	4	Interaction with substrate tRNA By similarity
Sites
	Site	101	1	Interaction with substrate tRNA By similarity
	Site	123	1	Interaction with substrate tRNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SM30 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 2B3181ABC60C3128	FASTA	300	35,076
	10 20 30 40 50 60 MKEDRIVFIF GPTAVGKSNI LFHFPRNKAE IINVDSIQVY KEFNIASSKP GKSLMKHIKH 70 80 90 100 110 120 HLVDFLEPEK EYTLGIFYEQ ALKIVKEIRQ KKKIPIFVGG TAFYFKHLKD GFPSTPLVTS 130 140 150 160 170 180 KIRIYVNNLL DLKGKSHLLK ELKNVDPIRF NMLNKNDIYR IKRSLEVYYQ TGIPISQFQK 190 200 210 220 230 240 KQNSEFKNIL IVGLKRSFED LKTRISIRIN EMLNSGLLSE IKGLFSKGYN ENTPAFKGIG 250 260 270 280 290 300 YNEFLLWKSR PWYSLNDIIS LINKNSVLYA KRQMTFFAKM PDVLWFHPED DLDDILNLIF

References

[1]

"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity." Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M. BMC Genomics 7:211-211(2006) [PubMed: 16914037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PKo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000395 Genomic DNA. Translation: ABH02098.1.
RefSeq	YP_710274.1. NC_008277.1.
3D structure databases
ProteinModelPortal	Q0SM30.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SM30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBORT00000003878; EBBORP00000003496; EBBORG00000003878.
GeneID	4227151.
GenomeReviews	Gene locus BAPKO_0874 in contig CP000395_GR.
KEGG	baf:BAPKO_0874.
NMPDR	fig|390236.5.peg.876.
PATRIC	37189995. VBIBorAfz3878_0878.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0324.
GeneTree	EBGT00050000007306.
HOGENOM	HBG559459.
OMA	HTRNYAK.
ProtClustDB	PRK14729.
Enzyme and pathway databases
BioCyc	BAFZ390236:BAPKO_0874-MONOMER.
Family and domain databases
HAMAP	MF_00185. IPP_trans. [Tree]
InterPro	IPR002627. IPPT. IPR018022. tRNA_delta_PyrP_Trfase. [Graphical view]
KO	K00791.
PANTHER	PTHR11088. IPPT. 1 hit.
Pfam	PF01715. IPPT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00174. MiaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MIAA_BORAP

Accession

Primary (citable) accession number: Q0SM30

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program