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Q0SM18 (SYI_BORAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:BAPKO_0886, BafPKo_0860
OrganismBorrelia afzelii (strain PKo) [Complete proteome] [HAMAP]
Taxonomic identifier390236 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group

Protein attributes

Sequence length1042 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Cofactor

Zinc By similarity. HAMAP-Rule MF_02003

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02003

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10421042Isoleucine--tRNA ligase HAMAP-Rule MF_02003
PRO_1000022148

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_02003
Motif594 – 5985"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site5971ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0SM18 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 51B78BF0D7E1C44F

FASTA1,042122,522
        10         20         30         40         50         60 
MFKKVENKAN FPKIEEKILK FWNDNKIFEK SMKQREGCEE FTFYDGPPFA TGLPHFGHFV 

        70         80         90        100        110        120 
PNTIKDIIPR YQTMRGKYVK RNFGWDTHGL PVEYEVEKKL GISGKYEIEN YGIENFNKEC 

       130        140        150        160        170        180 
KKIVLRYTEE WKNIILRLGR WVDFEKGYKT MDINFMESVW WVFKSLYNKG LIYESYYVLP 

       190        200        210        220        230        240 
YSPKLATPLS NFEVNLGEYK EVNDPSLTIK FKIKDKNEYL LAWTTTPWTL PSNLGIAVGQ 

       250        260        270        280        290        300 
EIEYSKIFDK KKEEILILGS KKLDSYYDDE NSYTIIEKFK GSKLEGIEYE PIFNYFLEQK 

       310        320        330        340        350        360 
DKGAFKVHMA DYVTTDDGTG IVHIAPFGEE DYRILKKHTN VDIIDPLDAE CKFTNRVKDF 

       370        380        390        400        410        420 
QGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNY PIIYRPISSW FVNVEKIKTK 

       430        440        450        460        470        480 
LLEVNEKINW MPAHLKKGRF GKWLENAKDW AISRNRFWGN PIPIWICSKT GKKICVGSKK 

       490        500        510        520        530        540 
ELESLSGQKI EDLHKDKVDK ITWPSKDGGT FIRTSEVLDC WFESGAMPYA SNHYPFTNES 

       550        560        570        580        590        600 
NFKNIFPADF IAEGLDQTRG WFYTLTILGV SLFESTAFKN VIVNGLVLSS DGRKMSKSFK 

       610        620        630        640        650        660 
NYTDPMEVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI WNAYSFFTTY 

       670        680        690        700        710        720 
AIIDKFQPPK NLNLVKNNNL DKWIISELES LKKILNNEID KYNLTKSIES LLEFIDKLNN 

       730        740        750        760        770        780 
WYIRRSRRRF WKSENDKDKN DAYETLYYAI KTLMILLAPF IPFITEEIYQ NLKTDEDKQS 

       790        800        810        820        830        840 
IHLNDYPKAN ENLINKTIEE KINLARKITS MARSLRSLHN IKIRMPISMI YIVTKNQNEQ 

       850        860        870        880        890        900 
NMLIEMQEII LDEINAKEMK IKSNEEDLIT YKAKANFKEL GKKLGKDMKT VSIEISKLKN 

       910        920        930        940        950        960 
EDIIKIINGI SYEIKVGNTK YYLSLNDIIL EREEKDNLKV INEESITIGI DSLITKELYL 

       970        980        990       1000       1010       1020 
EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENNETLQEIL NKFEKYIKTE TLALNIIFNK 

      1030       1040 
SKLEKKINLD DNIFTIIGIE KC 

« Hide

References

[1]"Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity."
Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M.
BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PKo.
[2]"Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates."
Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E.
J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PKo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000395 Genomic DNA. Translation: ABH02110.1.
CP002933 Genomic DNA. Translation: AEL70049.1.
RefSeqYP_005593039.1. NC_017238.1.
YP_710286.1. NC_008277.1.

3D structure databases

ProteinModelPortalQ0SM18.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING390236.BAPKO_0886.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABH02110; ABH02110; BAPKO_0886.
AEL70049; AEL70049; BafPKo_0860.
GeneID12158954.
4227163.
KEGGbaf:BAPKO_0886.
bafz:BafPKo_0860.
PATRIC37190021. VBIBorAfz3878_0891.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246403.
KOK01870.
OMADWNLSRS.
OrthoDBEOG644ZM1.
ProtClustDBPRK06039.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYI_BORAP
AccessionPrimary (citable) accession number: Q0SM18
Secondary accession number(s): G0IQM4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries