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Q0SM18

- SYI_BORAP

UniProt

Q0SM18 - SYI_BORAP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Borrelia afzelii (strain PKo)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei597 – 5971ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BAPKO_0886, BafPKo_0860
    OrganismiBorrelia afzelii (strain PKo)
    Taxonomic identifieri390236 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
    ProteomesiUP000001825: Chromosome, UP000005216: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10421042Isoleucine--tRNA ligasePRO_1000022148Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi390236.BAPKO_0886.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0SM18.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0SM18-1 [UniParc]FASTAAdd to Basket

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    MFKKVENKAN FPKIEEKILK FWNDNKIFEK SMKQREGCEE FTFYDGPPFA     50
    TGLPHFGHFV PNTIKDIIPR YQTMRGKYVK RNFGWDTHGL PVEYEVEKKL 100
    GISGKYEIEN YGIENFNKEC KKIVLRYTEE WKNIILRLGR WVDFEKGYKT 150
    MDINFMESVW WVFKSLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
    EVNDPSLTIK FKIKDKNEYL LAWTTTPWTL PSNLGIAVGQ EIEYSKIFDK 250
    KKEEILILGS KKLDSYYDDE NSYTIIEKFK GSKLEGIEYE PIFNYFLEQK 300
    DKGAFKVHMA DYVTTDDGTG IVHIAPFGEE DYRILKKHTN VDIIDPLDAE 350
    CKFTNRVKDF QGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNY 400
    PIIYRPISSW FVNVEKIKTK LLEVNEKINW MPAHLKKGRF GKWLENAKDW 450
    AISRNRFWGN PIPIWICSKT GKKICVGSKK ELESLSGQKI EDLHKDKVDK 500
    ITWPSKDGGT FIRTSEVLDC WFESGAMPYA SNHYPFTNES NFKNIFPADF 550
    IAEGLDQTRG WFYTLTILGV SLFESTAFKN VIVNGLVLSS DGRKMSKSFK 600
    NYTDPMEVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI 650
    WNAYSFFTTY AIIDKFQPPK NLNLVKNNNL DKWIISELES LKKILNNEID 700
    KYNLTKSIES LLEFIDKLNN WYIRRSRRRF WKSENDKDKN DAYETLYYAI 750
    KTLMILLAPF IPFITEEIYQ NLKTDEDKQS IHLNDYPKAN ENLINKTIEE 800
    KINLARKITS MARSLRSLHN IKIRMPISMI YIVTKNQNEQ NMLIEMQEII 850
    LDEINAKEMK IKSNEEDLIT YKAKANFKEL GKKLGKDMKT VSIEISKLKN 900
    EDIIKIINGI SYEIKVGNTK YYLSLNDIIL EREEKDNLKV INEESITIGI 950
    DSLITKELYL EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENNETLQEIL 1000
    NKFEKYIKTE TLALNIIFNK SKLEKKINLD DNIFTIIGIE KC 1042
    Length:1,042
    Mass (Da):122,522
    Last modified:September 5, 2006 - v1
    Checksum:i51B78BF0D7E1C44F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000395 Genomic DNA. Translation: ABH02110.1.
    CP002933 Genomic DNA. Translation: AEL70049.1.
    RefSeqiWP_011601260.1. NC_017238.1.
    YP_005593039.1. NC_017238.1.
    YP_710286.1. NC_008277.1.

    Genome annotation databases

    EnsemblBacteriaiABH02110; ABH02110; BAPKO_0886.
    AEL70049; AEL70049; BafPKo_0860.
    GeneIDi12158954.
    4227163.
    KEGGibaf:BAPKO_0886.
    bafz:BafPKo_0860.
    PATRICi37190021. VBIBorAfz3878_0891.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000395 Genomic DNA. Translation: ABH02110.1 .
    CP002933 Genomic DNA. Translation: AEL70049.1 .
    RefSeqi WP_011601260.1. NC_017238.1.
    YP_005593039.1. NC_017238.1.
    YP_710286.1. NC_008277.1.

    3D structure databases

    ProteinModelPortali Q0SM18.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 390236.BAPKO_0886.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABH02110 ; ABH02110 ; BAPKO_0886 .
    AEL70049 ; AEL70049 ; BafPKo_0860 .
    GeneIDi 12158954.
    4227163.
    KEGGi baf:BAPKO_0886.
    bafz:BafPKo_0860.
    PATRICi 37190021. VBIBorAfz3878_0891.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity."
      Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M.
      BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PKo.
    2. "Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates."
      Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E.
      J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PKo.

    Entry informationi

    Entry nameiSYI_BORAP
    AccessioniPrimary (citable) accession number: Q0SM18
    Secondary accession number(s): G0IQM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3