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Q0SM18

- SYI_BORAP

UniProt

Q0SM18 - SYI_BORAP

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BAPKO_0886, BafPKo_0860
Organism
Borrelia afzelii (strain PKo)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BAPKO_0886, BafPKo_0860
OrganismiBorrelia afzelii (strain PKo)
Taxonomic identifieri390236 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeBorreliaBorrelia burgdorferi group
ProteomesiUP000001825: Chromosome, UP000005216: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10421042Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022148Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi390236.BAPKO_0886.

Structurei

3D structure databases

ProteinModelPortaliQ0SM18.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0SM18-1 [UniParc]FASTAAdd to Basket

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MFKKVENKAN FPKIEEKILK FWNDNKIFEK SMKQREGCEE FTFYDGPPFA     50
TGLPHFGHFV PNTIKDIIPR YQTMRGKYVK RNFGWDTHGL PVEYEVEKKL 100
GISGKYEIEN YGIENFNKEC KKIVLRYTEE WKNIILRLGR WVDFEKGYKT 150
MDINFMESVW WVFKSLYNKG LIYESYYVLP YSPKLATPLS NFEVNLGEYK 200
EVNDPSLTIK FKIKDKNEYL LAWTTTPWTL PSNLGIAVGQ EIEYSKIFDK 250
KKEEILILGS KKLDSYYDDE NSYTIIEKFK GSKLEGIEYE PIFNYFLEQK 300
DKGAFKVHMA DYVTTDDGTG IVHIAPFGEE DYRILKKHTN VDIIDPLDAE 350
CKFTNRVKDF QGLFVKDADK KIIENLKLRN FLFKRENYLH RYPFCYRTNY 400
PIIYRPISSW FVNVEKIKTK LLEVNEKINW MPAHLKKGRF GKWLENAKDW 450
AISRNRFWGN PIPIWICSKT GKKICVGSKK ELESLSGQKI EDLHKDKVDK 500
ITWPSKDGGT FIRTSEVLDC WFESGAMPYA SNHYPFTNES NFKNIFPADF 550
IAEGLDQTRG WFYTLTILGV SLFESTAFKN VIVNGLVLSS DGRKMSKSFK 600
NYTDPMEVIN TFGADALRLY LIMSPVVKAD DLKYSDNGVR DVLKNIIIPI 650
WNAYSFFTTY AIIDKFQPPK NLNLVKNNNL DKWIISELES LKKILNNEID 700
KYNLTKSIES LLEFIDKLNN WYIRRSRRRF WKSENDKDKN DAYETLYYAI 750
KTLMILLAPF IPFITEEIYQ NLKTDEDKQS IHLNDYPKAN ENLINKTIEE 800
KINLARKITS MARSLRSLHN IKIRMPISMI YIVTKNQNEQ NMLIEMQEII 850
LDEINAKEMK IKSNEEDLIT YKAKANFKEL GKKLGKDMKT VSIEISKLKN 900
EDIIKIINGI SYEIKVGNTK YYLSLNDIIL EREEKDNLKV INEESITIGI 950
DSLITKELYL EGLTREFVRQ IQNLRKEKNF DVSDRINLYI ENNETLQEIL 1000
NKFEKYIKTE TLALNIIFNK SKLEKKINLD DNIFTIIGIE KC 1042
Length:1,042
Mass (Da):122,522
Last modified:September 5, 2006 - v1
Checksum:i51B78BF0D7E1C44F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000395 Genomic DNA. Translation: ABH02110.1.
CP002933 Genomic DNA. Translation: AEL70049.1.
RefSeqiWP_011601260.1. NC_017238.1.
YP_005593039.1. NC_017238.1.
YP_710286.1. NC_008277.1.

Genome annotation databases

EnsemblBacteriaiABH02110; ABH02110; BAPKO_0886.
AEL70049; AEL70049; BafPKo_0860.
GeneIDi12158954.
4227163.
KEGGibaf:BAPKO_0886.
bafz:BafPKo_0860.
PATRICi37190021. VBIBorAfz3878_0891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000395 Genomic DNA. Translation: ABH02110.1 .
CP002933 Genomic DNA. Translation: AEL70049.1 .
RefSeqi WP_011601260.1. NC_017238.1.
YP_005593039.1. NC_017238.1.
YP_710286.1. NC_008277.1.

3D structure databases

ProteinModelPortali Q0SM18.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 390236.BAPKO_0886.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABH02110 ; ABH02110 ; BAPKO_0886 .
AEL70049 ; AEL70049 ; BafPKo_0860 .
GeneIDi 12158954.
4227163.
KEGGi baf:BAPKO_0886.
bafz:BafPKo_0860.
PATRICi 37190021. VBIBorAfz3878_0891.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genome analysis: selection pressure on the Borrelia vls cassettes is essential for infectivity."
    Gloeckner G., Schulte-Spechtel U., Schilhabel M., Felder M., Suehnel J., Wilske B., Platzer M.
    BMC Genomics 7:211-211(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PKo.
  2. "Whole-genome sequences of two Borrelia afzelii and two Borrelia garinii Lyme disease agent isolates."
    Casjens S.R., Mongodin E.F., Qiu W.G., Dunn J.J., Luft B.J., Fraser-Liggett C.M., Schutzer S.E.
    J. Bacteriol. 193:6995-6996(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PKo.

Entry informationi

Entry nameiSYI_BORAP
AccessioniPrimary (citable) accession number: Q0SM18
Secondary accession number(s): G0IQM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 5, 2006
Last modified: September 3, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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