Reviewed, UniProtKB/Swiss-Prot Q0SJD2 (MHPB_RHOSR)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	RHA1_ro00518

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337668
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SJD2-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: FA31D55843964567	FASTA	314	33,844
	10 20 30 40 50 60 MPVALCTMSH SPLMGRNDPA QTVIDDVDAA FENARTFIAD FAPDLIVIFA PDHYNGVYYD 70 80 90 100 110 120 LMPPFCIGAA AQSVGDYGTE SGPLNVDRDA AYTVAREVLA SGVDVAFSER MHVDHGFAQA 130 140 150 160 170 180 LQLLVGSITA VPTVPIFINS VAEPLGPVSR VRLLGEAVGR AAANLDKRVL FVGSGGLSHD 190 200 210 220 230 240 PPVPQFATAP TEVKEKLIDG RNPTEAERNA REQRVIDAGR DFAAGVATIA PLNPEWDRNL 250 260 270 280 290 300 LDVLTSGEIE QIDSWTNEWF VEQAGHSSHE VRTWIAAYAA MSAAGKYRVT STFYREIPEW 310 IAGFGISTAV AVDE

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG92354.1.
RefSeq	YP_700512.1.
3D structure databases
SMR	Q0SJD2. Positions 5-307.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SJD2.
Genome annotation databases
GeneID	4218680.
GenomeReviews	Gene locus RHA1_ro00518 in contig CP000431_GR.
KEGG	rha:RHA1_ro00518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG42398.
HOGENOM	HBG565586.
OMA	PLNPEWD.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO00518-MONOMER.
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_RHOSR

Accession

Primary (citable) accession number: Q0SJD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 5, 2006
Last modified:	January 19, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)