SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q0SHW6

- BIOB_RHOSR

UniProt

Q0SHW6 - BIOB_RHOSR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Biotin synthase
Gene
bioB, RHA1_ro01043
Organism
Rhodococcus sp. (strain RHA1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi116 – 1161Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi149 – 1491Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi208 – 2081Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi278 – 2781Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRJOS101510:GJJ1-1042-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:RHA1_ro01043
OrganismiRhodococcus sp. (strain RHA1)
Taxonomic identifieri101510 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
ProteomesiUP000008710: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Biotin synthaseUniRule annotation
PRO_0000381589Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi101510.RHA1_ro01043.

Structurei

3D structure databases

ProteinModelPortaliQ0SHW6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0SHW6-1 [UniParc]FASTAAdd to Basket

« Hide

MTSAPVQTDI LALAREQVLE RGDALNESQV LEVLQLPDDR LEELLALAHD    50
VRMKWCGPEV EVEGIISLKT GGCPEDCHFC SQSGLFQSPV RAAWLDIPSL 100
VEAAKQTAKS GASEFCIVAA VRGPDERLLA QVAAGIEAIR NEVDIQIACS 150
LGMLTQEQVD QLAAMGVHRY NHNLETSKSH FPNVVTTHTW DERWNTLRMV 200
REAGMEVCCG GILGMGESLE QRAEFAANLA ELEPDEVPLN FLNPRPGTPF 250
GDLEVLPASE ALKSVAAFRL ALPRTILRFA GGREITLGDL GAKQGILGGI 300
NAVIVGNYLT TLGRPAEQDL DLLVDLQMPI KALNDTL 337
Length:337
Mass (Da):36,524
Last modified:July 28, 2009 - v2
Checksum:iC8BD3EC375958CDE
GO

Sequence cautioni

The sequence ABG92870.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000431 Genomic DNA. Translation: ABG92870.1. Different initiation.
RefSeqiYP_701028.1. NC_008268.1.

Genome annotation databases

EnsemblBacteriaiABG92870; ABG92870; RHA1_ro01043.
GeneIDi4219281.
KEGGirha:RHA1_ro01043.
PATRICi23200891. VBIRhoJos26306_1065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000431 Genomic DNA. Translation: ABG92870.1 . Different initiation.
RefSeqi YP_701028.1. NC_008268.1.

3D structure databases

ProteinModelPortali Q0SHW6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 101510.RHA1_ro01043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABG92870 ; ABG92870 ; RHA1_ro01043 .
GeneIDi 4219281.
KEGGi rha:RHA1_ro01043.
PATRICi 23200891. VBIRhoJos26306_1065.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci RJOS101510:GJJ1-1042-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RHA1.

Entry informationi

Entry nameiBIOB_RHOSR
AccessioniPrimary (citable) accession number: Q0SHW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi