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Q0SHW6

- BIOB_RHOSR

UniProt

Q0SHW6 - BIOB_RHOSR

Protein

Biotin synthase

Gene

bioB

Organism
Rhodococcus sp. (strain RHA1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi116 – 1161Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi149 – 1491Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi208 – 2081Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi278 – 2781Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRJOS101510:GJJ1-1042-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:RHA1_ro01043
    OrganismiRhodococcus sp. (strain RHA1)
    Taxonomic identifieri101510 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeRhodococcus
    ProteomesiUP000008710: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337Biotin synthasePRO_0000381589Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi101510.RHA1_ro01043.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0SHW6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0SHW6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSAPVQTDI LALAREQVLE RGDALNESQV LEVLQLPDDR LEELLALAHD    50
    VRMKWCGPEV EVEGIISLKT GGCPEDCHFC SQSGLFQSPV RAAWLDIPSL 100
    VEAAKQTAKS GASEFCIVAA VRGPDERLLA QVAAGIEAIR NEVDIQIACS 150
    LGMLTQEQVD QLAAMGVHRY NHNLETSKSH FPNVVTTHTW DERWNTLRMV 200
    REAGMEVCCG GILGMGESLE QRAEFAANLA ELEPDEVPLN FLNPRPGTPF 250
    GDLEVLPASE ALKSVAAFRL ALPRTILRFA GGREITLGDL GAKQGILGGI 300
    NAVIVGNYLT TLGRPAEQDL DLLVDLQMPI KALNDTL 337
    Length:337
    Mass (Da):36,524
    Last modified:July 28, 2009 - v2
    Checksum:iC8BD3EC375958CDE
    GO

    Sequence cautioni

    The sequence ABG92870.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000431 Genomic DNA. Translation: ABG92870.1. Different initiation.
    RefSeqiYP_701028.1. NC_008268.1.

    Genome annotation databases

    EnsemblBacteriaiABG92870; ABG92870; RHA1_ro01043.
    GeneIDi4219281.
    KEGGirha:RHA1_ro01043.
    PATRICi23200891. VBIRhoJos26306_1065.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000431 Genomic DNA. Translation: ABG92870.1 . Different initiation.
    RefSeqi YP_701028.1. NC_008268.1.

    3D structure databases

    ProteinModelPortali Q0SHW6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 101510.RHA1_ro01043.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABG92870 ; ABG92870 ; RHA1_ro01043 .
    GeneIDi 4219281.
    KEGGi rha:RHA1_ro01043.
    PATRICi 23200891. VBIRhoJos26306_1065.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci RJOS101510:GJJ1-1042-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RHA1.

    Entry informationi

    Entry nameiBIOB_RHOSR
    AccessioniPrimary (citable) accession number: Q0SHW6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3