Q0SHV6 (BIOD_RHOSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	RHA1_ro01053

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302542
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
	Nucleotide binding	109 – 112	4	ATP By similarity
	Nucleotide binding	200 – 202	3	ATP By similarity
Sites
	Metal binding	12	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	50	1	Magnesium 2 By similarity
	Metal binding	109	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	50	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SHV6 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 3287C064D24AF150	FASTA	231	23,047
	10 20 30 40 50 60 MSILVVTGTS TDVGKTVVTA ALAAAAREAG LSVAVCKPAQ TGVAPGGPGD LAEVTRLSGV 70 80 90 100 110 120 TAVVELARYP EPLAPDTAAR RSGLPMLRCA DVVETVRALD DGHDLVLVEG AGGLLVRLGA 130 140 150 160 170 180 EGFTLVDLAR ALDAPAVVVA AAGLGTLNHT ELTIRALSAA GLACAGTVIG SWPDEPGLAE 190 200 210 220 230 RCNLDDLPAV TGVDVVGSVP AGSGRLASAS FGAAASTWFD STWLKSTLTR L

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RHA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG92880.1.
RefSeq	YP_701038.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0SHV6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SHV6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4219563.
GenomeReviews	Gene locus RHA1_ro01053 in contig CP000431_GR.
KEGG	rha:RHA1_ro01053.
PATRIC	23200913. VBIRhoJos26306_1076.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	AEAVGWC.
PhylomeDB	Q0SHV6.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO01053-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_RHOSR

Accession

Primary (citable) accession number: Q0SHV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program