Q0SH52 (PROB_RHOSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	RHA1_ro01310

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Glutamate 5-kinase HAMAP MF_00456		PRO_0000252994
Regions
	Domain	278 – 352	75	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q0SH52 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: DE6FD7BB4B1259BA	FASTA	366	37,538
	10 20 30 40 50 60 MSATRRTIAS AGSIVVKIGS SALTSLVGGL DVGRLDALAD AIEDRMRAGS DVVVVSSGAV 70 80 90 100 110 120 GAGLAPLGLT KRPRDLATKQ AAASVGQLAL AHAWGTSFAR YGRTVGQVLL TADDIARRAQ 130 140 150 160 170 180 HRNAQRTLDR LRALHAVAIV NENDTVATAE LRFGDNDRLA ALVAHLVGAD ALVLLSDVDG 190 200 210 220 230 240 LYDGDPRKGN ATLIPEVNSP EDLDGVVAGS GGALGTGGMA SKLSAARLAA DAGVPVLLAA 250 260 270 280 290 300 ASDAGAALRD AGVGTAFVAR PSRLSARKFW VRHAADEQGI LHIDEGAVRA VVTKRRSLLP 310 320 330 340 350 360 AGISAVSGRF YGGDVVSLLG PDERPVARGV VAYDSAEISD ILGKSTQELP AEMQRPVVHA DDLVPL

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RHA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG93134.1.
RefSeq	YP_701292.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0SH52.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SH52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4219164.
GenomeReviews	Gene locus RHA1_ro01310 in contig CP000431_GR.
KEGG	rha:RHA1_ro01310.
PATRIC	23201441. VBIRhoJos26306_1337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HBG507643.
OMA	TFGDNDM.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO01310-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_RHOSR

Accession

Primary (citable) accession number: Q0SH52

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program