Q0SGZ0 (CLPP2_RHOSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 2
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 2

Gene names

Name:	clpP2
Ordered Locus Names:	RHA1_ro01372

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444		PRO_0000252839
Sites
	Active site	92	1	By similarity
	Active site	117	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SGZ0 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 6B9B70790F7F693F	FASTA	195	20,849
	10 20 30 40 50 60 MTSATAGLNL SDSVYERLLR ERIIFLGTQV DDDIANKLCA QILLLSAEDP SRDISLYINS 70 80 90 100 110 120 PGGSVSAGMA IFDTMQFAEC DVATFGMGLA ASMGQFLLSA GAKGKRYALP HARIMMHQPS 130 140 150 160 170 180 AGIGGTASDI AIMAEQFAHT KREMAELIAE HTGQTVEQIT ADSDRDRWFT AQQALEYGFV 190 DHVVSRAAQA GGTGQ

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RHA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG93196.1.
RefSeq	YP_701354.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0SGZ0.
SMR	Q0SGZ0. Positions 9-187.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SGZ0.
Protein family/group databases
MEROPS	S14.008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4219709.
GenomeReviews	Gene locus RHA1_ro01372 in contig CP000431_GR.
KEGG	rha:RHA1_ro01372.
PATRIC	23201565. VBIRhoJos26306_1399.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0740.
HOGENOM	HBG558421.
OMA	NAICAQL.
ProtClustDB	PRK00277.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO01372-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP2_RHOSR

Accession

Primary (citable) accession number: Q0SGZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program