Q0SGR9 (GLGB_RHOSR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	RHA1_ro01449

Organism

Rhodococcus sp. (strain RHA1) [Complete proteome] [HAMAP]

Taxonomic identifier

101510 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000260687
Sites
	Active site	408	1	Nucleophile By similarity
	Active site	461	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0SGR9 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: F9A397E08C0D7BF4	FASTA	732	81,651
	10 20 30 40 50 60 MTVEPPVAIA PDAADLDLLS RGEHHNPHSI LGAHPHPDGT VIRALRPHAE AVDAVIGGTS 70 80 90 100 110 120 FSLEHLAHGV WGALVPYRDL MDYRLSTTWP GGHNDVSADG YRFLPTLGEL DLHLFGEGRH 130 140 150 160 170 180 ERLWEILGAH RRRYDTPDGT VTGTSFAVWA PNARGVSVIG DFDGWSGRNY PMRVLGSTGV 190 200 210 220 230 240 WELFVPGIEA GDLYKFRVHG PDGSVRDKAD PMAFATEVPP ATASRVSVST YEWNDAEWLA 250 260 270 280 290 300 QRAATEPAQS PMSVYEVHLA SWRPGLNYRE MAEQLAVHLT ETGFTHVELL PVAEHPFGGS 310 320 330 340 350 360 WGYQVTSYYA PTSRFGSPDD FRWFVDHLHA AGIGVIVDWV PAHFPKDEWA LARFDGTPLY 370 380 390 400 410 420 EHSDPQRGEQ LDWGTYVFDF GRREVRNFLV ANALYWLDEF HVDGLRVDAV ASMLYLDYSR 430 440 450 460 470 480 PEGGWTPNIH GGRENLEAVA FLQETNATVH KQHRGVVTIA EESTAWPGVT RATNVGGLGF 490 500 510 520 530 540 NMKWNMGWMH DTLGFMAHDP VHRSYHHHEI TFSLMYAWSE NYLLPISHDE VVHGKGTLWT 550 560 570 580 590 600 RMPGDDYAKA AGVRALLAYM WSHPGKQLLF MGQEFGQTAE WSEERGLDWY QLDDPYTGGF 610 620 630 640 650 660 HRGLLRLVHD LNATYREHPA LWTLDTSPGG FSWIDANDTA NNVLSFLRYG TDGSILACLF 670 680 690 700 710 720 NFSGSPHANY RVGLPERGEW REILNTDAEI YAGSGWGNLG AVTATSQPWH GRPASAEVAL 730 PANGAIWMSL ER

References

[1]

"The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse." McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C., Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H., Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H. , Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R., Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W., Eltis L.D. Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006) [PubMed: 17030794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RHA1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000431 Genomic DNA. Translation: ABG93267.1.
RefSeq	YP_701425.1. NC_008268.1.
3D structure databases
ProteinModelPortal	Q0SGR9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0SGR9.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4219230.
GenomeReviews	Gene locus RHA1_ro01449 in contig CP000431_GR.
KEGG	rha:RHA1_ro01449.
PATRIC	23201721. VBIRhoJos26306_1471.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	DGTCLYE.
PhylomeDB	Q0SGR9.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	RSP101510:RHA1_RO01449-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_RHOSR

Accession

Primary (citable) accession number: Q0SGR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program